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SNL 76/7

SNL 76/7

Catalogue No.

07032801

Cell Line Name

SNL 76/7

Cell Line Description

SNL 76/7 clonally-derived from a STO cell line that expresses both G418 resistance and leukaemic inhibitory factor (LIF). The STO cell line was transfected with a G418-resistance cassette, RV4.0 (Thomas and Capecchi 1987), and a LIF expression construct (Williams et al., 1988). This cell line can be used as a feeder layer to support the growth of mouse embryonic stem cells. Inclusion of G418 in the media is not necessary for normal cell growth.

General Info

Species

Mouse

Release Conditions

Restricted - commercial organisations are required to complete the 'Cell Line Release Authorisation for Research Use in Commercial Organisations' release conditions form in the supporting documents section.

Characteristics

Tissue of Origin

Embryo

Karyotype

Not specified

Disease

None Stated

Culture Conditions

Cell Type

Fibroblastic

Subculture Routine

Split confluent cultures 1:5-1:15 i.e. seeding at 2-4x10,000 cells/cm² using trypsin/EDTA; 5% CO₂; 37°C.

Culture Medium

Knockout D-MEM (Invitrogen) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)

Growth Mode

Adherent

Additional Info

Depositor

Profesor Allan Bradley, The Sanger Centre, Wellcome Trust Genome Campus, Hinxton, UK

Country of Origin

United Kingdom

GMO Status

Genetically Modified Organism Class 1 (GMO1)

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Thomas and Capecchi (1987) Site-directed mutagenesis by gene targeting in mouse embryo-derived stem cells. Cell 51(3): 503-12.

Bibliography

Williams et al., (1988) Myeloid leukaemia inhibitory factor maintains the developmental potential of embryonic stem cells. Nature 336(6200): 684-7.

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: SNL 76/7 (ECACC 07032801).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.