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UK Health Security Agency

SSE-5

SSE-5

Catalogue No.

95122021

Cell Line Name

SSE-5

Cell Line Description

SSE-5 was initiated in 1964 from pooled embryos of Sockeye Salmon (Oncorynchus nerka). The cell line that is also known as Sockeye Salmon embryo-5, is susceptible to infectious pancreatic necrosis virus (IPNV), viral hemorrhagic septicaemia virus (VHSV) and infectious hematopoietic necrosis virus (IHNV). For replication of salmonid viruses SSE-5 cells should be grown at 18°C.

Characteristics

Tissue of Origin

Embryo

Karyotype

Modal no. 55

Applications

Isolation of viruses from clinical samples, virus propagation

Disease

None Stated

Culture Conditions

Cell Type

Epithelial-like

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 15-21°C; split every 10-14 days. Fish cell lines detach easily during transit if the culture is very young. After resuscitation split cells 1:3 and culture for at least one week with 1-2 media changes before shipping.

Culture Medium

MEM (EBSS) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Growth Mode

Adherent

Additional Info

Depositor

Dr J L Fryer, Department of Microbiology, Oregon State University, Corvallis, USA

Country of Origin

United States

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

In Vitro 1984; 20:671

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: SSE-5 (ECACC 95122021).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.