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UK Health Security Agency

SW 626

SW 626

Catalogue No.

91091203

Cell Line Name

SW 626

Cell Line Description

Although originally thought to be of ovarian origin, a recent report has suggested that the SW 686 cell line might have been derived from an ovarian metastasis of a primary adenocarcinoma of the colon Furlong, et al., 1999. The SW 626 cell line was initiated by A. Leibovitz in January 1974 at the Scott and White Clinic, Temple, Texas from a surgical specimen from a cystadenocarcinoma of the ovary in a 46 year old female Caucasian. The histopathology of the specimen was determined to be grade III adenocarcinoma.

Characteristics

Tissue of Origin

Ovary

Karyotype

Hypertetraploid; modal no. 104

Disease

Adenocarcinoma

Culture Conditions

Cell Type

Epithelial

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-3x10,000 cells/cm² using 0.05% trypsin/EDTA; CO₂ is not needed. Medium change after 4-5 days. 37°C.

Culture Medium

L15 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Growth Mode

Adherent

Additional Info

Depositor

Obtained from ATCC

Country of Origin

United States

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Furlong et al., (1999) Evidence for the colonic origin of ovarian cancer cell line SW626. J. Natl. Cancer Inst. 91: 1327-1328.

Bibliography

Fogh et al., (1977) Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214. Fogh et al., (1977) One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226. Strobel et al., (1996) BAX enhances paclitaxel-induced apoptosis through a p53-independent pathway. Proc. Natl. Acad. Sci. USA 93: 14094-14099.

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: SW 626 (ECACC 91091203).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.