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UK Health Security Agency

T02J-7/10

T02J-7/10

Catalogue No.

10031603

Cell Line Name

T02J-7/10

Cell Line Description

T02J-7/10 is clonally-derived from a CHO-K1 cell line which has been transfected with a human cholinergic receptor muscarinic M3 G Protein Coupled Receptor (GPCR) cassette to allow expression of the human M3 GPCR.T02J-7/10 is an example of a cell line transfected using InSCREENeX SCREENflexTM recombinase mediated cassette exchange (RMCE) methodology (see www.inscreenex.com).

General Info

Species

Hamster

Also Known As

T02J-7/10 (CHO-M3 (CHRM3))

Release Conditions

Restricted – all customers must complete the specific depositor MTA.

Characteristics

Receptors

Human cholinergic receptor muscarinic M3 (CHRM3)

Products

Not specified

Tissue of Origin

Ovary

Karyotype

Not specified

Applications

Drug screening

Disease

None Stated

Culture Conditions

Cell Type

Epithelial

Subculture Routine

Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 2 x 10⁴ cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Culture Medium

Ham's F12 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Growth Mode

Adherent

Additional Info

Depositor

Dr Roland Schucht, InSCREENeX GmbH, Inhoffenstrasse 7, 38124 Braunschweig, Germany

Country of Origin

Germany

GMO Status

Genetically Modified Organism Class 1 (GMO1)

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Schucht R, Coroadinha AS, Zanta-Boussif MA, Verhoeyen E, Carrondo MJ, Hauser H, Wirth D. (2006) A new generation of retroviral producer cells: predictable and stable virus production by Flp-mediated site-specific integration of retroviral vectors. Mol Ther. 14(2):285-92 PMID: 16697259.

Bibliography

Nehlsen K, Schucht R, da Gama-Norton L, Krömer W, Baer A, Cayli A, Hauser H, Wirth D. (2009) Recombinant protein expression by targeting pre-selected chromosomal loci. BMC Biotechnol. 9:100 PMID: 20003421. Schucht R, Wirth D, May T. (2010) Precise regulation of transgene expression level and control of cell physiology. Cell Biol Toxicol. 26(1):29-42 PMID: 19763851.

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: T02J-7/10 (ECACC 10031603).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.