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UK Health Security Agency

TAK3

TAK3

Catalogue No.

93093004

Cell Line Name

TAK3

Cell Line Description

Peripheral blood lymphocytes from a healthy female Tunisian immortalised by EBV transformation. The cell line contains the following deletion, at the homozygous state, in the human immunoglobulin heavy locus (IGH on chromosome 14 at 14q32.33), of 6 IGHC genes: on both chromosomes 14, deletion I (del G1-EP1-A1-GP-G2-G4). The Gm-Am genotype is Gm5*; - ; - A2m2/Gm5*; - ; - A2m2 (Gm b*; - ; - A2m2/Gm b*; - ; - A2m2). Gm allotypes are indicated in the order G3m; G1m; G2m. The absence of G1m and G2m allotypes due to the deletion of the IGHG1 and IGHG2 genes is indicated by (-). An Epstein-Barr-transformed lymphoblastoid cell line with an identical deletion I, at the homozygous state, is available, from one member of another Tunisian family, as EZZ (TOU II-4) (93093003).

Characteristics

Tissue of Origin

Blood

Karyotype

Not specified

Disease

None Stated

Culture Conditions

Cell Type

Lymphoblastoid

Subculture Routine

Maintain cultures at 5-9x100,000 cells/ml; 5% CO₂; 37°C.

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Growth Mode

Suspension

Additional Info

Depositor

Prof M-P Lefranc and Prof G Lefranc, Laboratoire d'ImmunoGenetique Moleculaire (LIGM), Universite de Montpellier

Country of Origin

France

GMO Status

Genetically Modified Organism Class 1 (GMO1)

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Lefranc M-P, Lefranc G, Rabbitts TH. 1982 Inherited deletion of immunoglobulin heavy chain constant region genes in normal human individuals. Nature 300(5894):760-2. PMID: 6817143

Bibliography

Lefranc M-P, Lefranc G. 1987 Human immunoglobulin heavy-chain multigene deletions in healthy individuals. FEBS Letters 213(2):231-7. PMID: 3104087

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: TAK3 (ECACC 93093004).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.