TK6 (IVGT)
TK6 (IVGT)
Cell Line Name
Cell Line Description
JCRBNo 1435, lot number 11032011. This line is a derivative of the WIL-2 lymphoblast cell line. The cells are heterozygous at the thymidine (TK) kinase locus and can be used to quantitatively detect forward mutations at 3 loci.
General Info
Species
Human
Links
Cellosaurus: CVCL_AR68
Release Conditions
Characteristics
Receptors
Products
Tissue of Origin
Morphology
Applications
Mutagenesis assays
Disease
Culture Conditions
Cell Type
Subculture Routine
Dilute cell in fresh medium at 0.1- 1.5 x 10⁵ cells per ml.
Culture Medium
RPMI1640 + 10% Horse Serum + 2mM L-Glutamine+ 1.8 mM Sodium Pyruvate. Cryopreserve cells in culture medium with cells heat-inactivated 10% Horse Serum and 10% Dimethyl sulphoxide (DMSO)
Growth Mode
Additional Info
Depositor
Country of Origin
Hazard Group (ACDP)
Applications
References
Skopek TR, Liber HL, Penman BW, Thilly WG. Isolation of a human lymphoblastoid line heterozygous at the thymidine kinase locus: possibility for a rapid human cell mutation assay. Biochem. Biophys. Res. Commun. 1978 Sep 29;84(2):411-6. PubMed PMID: 214074
Available Formats
- Frozen
- DNA-5µg (100ng/µl)
Citation Guidance
If use of this culture results in a scientific publication, it should be cited in the publication as: TK6 (IVGT) (ECACC 13051501).
Biosafety Information
Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.
Further Information
The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.
Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.