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UK Health Security Agency

TKE2

TKE2

Catalogue No.

11033107

Cell Line Name

TKE2

Cell Line Description

TKE2 is the first cell line of mouse corneal epithelial progenitor cells isolated from the basal layer of the limbal epithelium.TKE2 has applications in the cell biology of corneal epithelial cells, toxicity, and differentiation. TKE2 can be induced to differentiate into stratified epithelium on increasing the calcium concentration and addition of foetal bovine serum.

Characteristics

Receptors

Not specified

Products

Stains positive for nuclear p63 expression and K14 keratin.

Tissue of Origin

Cornea

Morphology

Small and regular cobblestone-like shape

Karyotype

Normal karyotype of the female mouse CD1 strain

Disease

None Stated

Culture Conditions

Cell Type

Epithelial

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 2 x 102 - 2 x 10⁴ cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C. It is important to inactivate the trypsin with an equal volume of trypsin inhibitor. To remove all traces of trypsin and trypsin inhibitor pellet the cells by centrifugation and resuspend the cells in fresh medium. Media change every second day.

Culture Medium

Stemline Keratinocyte Medium II (Sigma S0196) + Stemline Keratinocyte Growth Supplement (Sigma S9945) + 2mM Glutamine.

Growth Mode

Adherent

Additional Info

Depositor

Dr Tetsuya Kawakita, Shinanomachi35 , Shinjuku, Tokyo, Japan 160-8583

Country of Origin

Japan

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Kawakita T, Shimmura S, Hornia A, Higa K, Tseng SC. 2008 Stratified epithelial sheets engineered from a single adult murine corneal/limbal progenitor cell. J Cell Mol Med. 12(4):1303-16 PMID: 18318692.

Bibliography

Kawakita T, Espana EM, He H, Yeh LK, Liu CY, Tseng SC 2004 Calcium-induced abnormal epidermal-like differentiation in cultures of mouse corneal-limbal epithelial cells. Invest Ophthalmol Vis Sci. 45(10):3507-12 PMID: 15452056. Wang H, Tao T, Tang J, Mao YH, Li W, Peng J, Tan G, Zhou YP, Zhong JX, Tseng SC, Kawakita T, Zhao YX, Liu ZG 2009 Importin 13 serves as a potential marker for corneal epithelial progenitor cells. Stem Cells. 27(10):2516-26 PMID: 19593795.

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: TKE2 (ECACC 11033107).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.