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UK Health Security Agency

TPS

TPS

Catalogue No.

96112839

Cell Line Name

TPS

Cell Line Description

TPS has been initiated from a long-term pronephric stroma culture of an adult 1 year old rainbow trout (Oncorhynchus mykiss W). The cells grow at 4° to 22°C, with an optimum temperature of 18°C. They do not form true monolayers, but overlap in a multilayered network of fibroblastic, epithelioid and giant cells. TPS cells are susceptible to viral haemorrhagic septicemia virus (VHSV) and infectious haemopoietic necrosis virus (IHNV). They have been applied to support the development of haemopoiesis when co-cultured with haemopoietic precursors. Spontaneous angiogenesis processes in vitro occurred in TPS cultures when they are kept in the same flask, i.e. without passages, for long times (over 2 months).

Characteristics

Tissue of Origin

Kidney (anterior)

Karyotype

Modal no. 58

Applications

Fish Haemopoiesis and Fish pathology

Disease

None Stated

Culture Conditions

Cell Type

Epithelial-like/Fibroblast-like

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:4 using 0.05% trypsin or trypsin/EDTA; trypsinise at room temperature once a week; air atmosphere; 18°C. Fish cell lines detach easily during transit if the culture is very young. After resuscitation split cells 1:3 to 1:4 and culture for at least one week with 1-2 media changes before shipping.

Culture Medium

At ECACC successful growth of this cell line has been achieved using MEM alpha modification + 10% FBS + 2mM Glutamine. Previously these cells were grown in RPMI 1640 + 2mM Glutamine + 25mM Hepes + 2.5mM NaP + 0.01mM Hydrocortisone-21-hemisuccinate + 0.088mM Guanosine + 0.0225mM Cytidine + 0.094mM Adenosine + 0.102mM Uridine + 10% FBS + 10% HS, pH 7.4-7.6.

Growth Mode

Adherent

Additional Info

Depositor

Professor A J Villena, Department of Cellular Biology, University of Leon, Spain

Country of Origin

Spain

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Fish and Shellfish Immunology 1995;5:441

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: TPS (ECACC 96112839).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.