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UK Health Security Agency

UKRV-Mel-17

UKRV-Mel-17

Catalogue No.

13012514

Cell Line Name

UKRV-Mel-17

Cell Line Description

UKRV-Mel-17 (ESTDAB-147) was established from the lymph node metastasis in a 48 year-old male patient with metastatic melanoma (skin stage IV). The UKRV-Mel-17 cell line is one of more than 170 characterized melanoma cell lines collected during the ESTDAB project (European Searchable Tumour Line Database) part of the European Commission's fifth framework infrastructures programme (contract no. QLRI-CT-2001-01325). ESTDAB (http://www.ebi.ac.uk/ipd/estdab) contains information on HLA genotype and surface expression of HLA molecules, expression of tumour antigens, antigen processing capability, production of and response to cytokines and chemokines, apoptosis regulation and expression of adhesion/accessory molecules. This information is appended below. Such information allows researchers to identify cells possessing specific parameters important for studies of immunity, immunogenetics, gene expression, metastasis, response to chemotherapy, and other tumour biological experimentation. Robinson J, Waller MJ, Fail SC, Marsh SG. 2006 The IMGT/HLA and IPD databases. Hum Mutat 27(12):11929.PMID: 16944494. Pawelec G, Marsh SG. 2006 ESTDAB: a collection of immunologically characterised melanoma cell lines and searchable databank. Cancer. Immunol Immunother. 55(6):623-7.PMID: 16421722. Robinson J, Roberts CH, Dodi IA, Madrigal JA, Pawelec G, Wedel L, Marsh SG.2009 The European searchable tumour line database. Cancer. Immunol Immunother. 58(9):1501-6. PMID: 19172270.

Characteristics

Receptors

see http://www.ebi.ac.uk/ipd/estdab

Products

see http://www.ebi.ac.uk/ipd/estdab

Tissue of Origin

Lymph node

DNA profile (STR Profile)

Amelogenin: X,Y
CSF1PO: 10,11
D5S818: 11,12
D7S820: 8
D13S317: 11
D16S539: 10,13
TH01: 6
TPOX: 8,11
vWA: 15,16

Disease

Melanoma

Culture Conditions

Cell Type

Epithelial

Subculture Routine

Split sub-confluent cultures (70-80%) seed new cultures at 1-4x10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Saturation density approx 8x10,000 cells/cm².

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Growth Mode

Adherent

Additional Info

Depositor

Prof D Schadendorf, Department of Dermatology, University of Duisburg-Essen, Essen, Germany.

Country of Origin

Germany

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Horn S, Figl A, Rachakonda PS, Fischer C, Sucker A, Gast A, Kadel S, Moll I, Nagore E, Hemminki K, Schadendorf D, Kumar R.2013 TERT promoter mutations in familial and sporadic melanoma. Science. 339(6122):959-61. PMID: 23348503

Bibliography

Ugurel S, Thirumaran RK, Bloethner S, Gast A, Sucker A, Mueller-Berghaus J, Rittgen W, Hemminki K, Becker JC, Kumar R, Schadendorf D. 2007 B-RAF and N-RAS mutations are preserved during short time in vitro propagation and differentially impact prognosis. PLoS One. 2(2):e236.PMID: 17311103.

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: UKRV-Mel-17 (ECACC 13012514).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.