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VA-ES-BJ

VA-ES-BJ

Catalogue No.

94092303

Cell Line Name

VA-ES-BJ

Cell Line Description

Spontaneously transformed cells from a male patient with epithelioid sarcoma derived from bone marrow metastasis. Cells grow very rapidly and secrete GM-CSF. They have been shown to express AE-1 and AE-3 cytokeratins, epithelial membrane antigen, p53, human Fas and vimentin. VA-ES-BJ cells are sensitive to oxidising agents, busulfaxamine and protoporphyrin IX. Epithelial, spindle and giant cells.

Characteristics

Receptors

Granulocyte Macrophage Colony Stimulating Factor (GM-CSF), AE, AE3, Fas, epithelial membrane antigen (EMA), Vimentin, P53

Products

Granulocyte Macrophage Colony Stimulating Factor (GM-CSF)

Tissue of Origin

Skin (bone marrow metastasis)

DNA profile (STR Profile)

Amelogenin: X
CSF1PO: 11,12
D5S818: 11,13
D7S820: 11,12
D13S317:11,12
D16S539: 11,12
TH01: 6
TPOX: 8
vWA: 18,19

Karyotype

66-68 Aneuploidic, pentasomy #7, multiple trisomy

Applications

Study of tumours Tumourigenic in nude mice

Disease

Sarcoma

Culture Conditions

Cell Type

Epithelial

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.05% trypsin/EDTA; 5% CO₂; 37°C.

Culture Medium

DMEM + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Growth Mode

Adherent

Additional Info

Depositor

Mrs C Helson, St Agnes Hospital, 305 North St, White Plains, New York

Country of Origin

United States

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Int J Oncology 1995;7:51

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: VA-ES-BJ (ECACC 94092303).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.