Catalogue No.
19072511
WCB
WCB
Cell Line Name
WCB
Cell Line Description
The cell line hybridoma WCB was developed by immunising BALB/c mice with detergent-insoluable Trypanosoma brucei cytoskeletons.
Trypanosoma brucei is a protozoan parasite that belongs to the order Kinetoplastida, which is responsible for a wide range of diseases affecting humans and cattle in Africa.
Mouse (BALB/c) spleen cells from above were fused with the myeloma Sp2-0/Ag14.
General Info
Fusion Species
Mouse x Mouse
Target (Reactivity)
Anti-microtubule cross bridges. It detects an antigen (WCB210) associated specifically with the sub-pellicular microtubules and not with the flagellum microtubules.
Also known as
WCB-1, Whole cell body-1
Unique to ECACC
Yes
Nagoya Status
Not Applicable
Release Conditions
Characteristics
Myeloma
Sp2/0-Ag14
Immunogen
Trypanosoma brucei cytoskeletons
Antibody Isotype
This was deposited as IgG, ECACC tested it as part of the QC process and it was found to be IgG1.
Morphology
Spherical
Tissue of Origin
Hybrid
Culture Conditions
Cell Type
Hybridoma
Subculture Routine
Maintain cultures between 1-9 x 100,000 cells/ml; 5% CO2; 37°C
On resuscitation, a centrifugation step to remove the cryoprotectant is essential. Rapidly thaw the frozen ampoule in a water bath at 37°C for 1-2 minutes. Transfer the contents to a centrifuge tube and slowly add 5-10ml of pre-warmed growth media. Remove a sample for counting. Centrifuge at 100 x g for 2-3 minutes to pellet cells and seed at a relatively high density of 5-7 x 10⁵ cells/ml.
Place culture flask flat and observe regularly until viable proliferating cells are seen. When recovering hybridoma cultures from frozen it is not unusual for growth to be slower than expected initially and there may be an observed decrease in viability.
Establishment of an actively proliferating culture may take up to 2 weeks.
Culture Medium
DMEM + 2mM glutamine + 10% FBS
Often hybridoma cultures may benefit from being re-suspended with media supplemented with 20% FBS in the early critical stage of culture establishment immediately post resuscitation. Once the culture is established in culture the FBS can be reduced to 10%.
Growth Mode
Suspension
Additional Info
Depositor
Keith Gull, University of Oxford
Country of Origin
United Kingdom
Applications
Western blot, Immonoblotting, Immunofluorescence
GMO Status
Not Applicable
Hazard Group (ACDP)
2
References
References
Woods, A., Sherwin, T., Sasse, R., MacRae, T.H., Baines, A.J. and Gull, K. (1989) Definition of individual components within the cytoskeleton of Trypanosoma brucei by a library of monoclonal antibodies. Journal of Cell Science 93: 491-500.
Woods, A., Baines, A amd Gull, K. (1992) A high molecular mass phosphoprotein defined by a novel monoclonal antibody is closely associated with the intermicrotubule cross bridges in the Trypanosoma brucei cytoskeletonJ Cell Sci 103 (3): 665–675.
Baines, A. and Gull, K., (2008) WCB is a C2 domain protein defining the plasma membrane - sub-pellicular microtubule corset of kinetoplastid parasites. Protist. 159(1): 115-25.
Available Formats
- Frozen
Citation Guidance
If use of this culture results in a scientific publication, it should be cited in the publication as: WCB (ECACC 19072511)
Biosafety Information
Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country.
ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.
Further Information
The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.
Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.