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WES

WES

Catalogue No.

91011612

Cell Line Name

WES

Cell Line Description

A non-producer, Adeno 12-SV40 transformed chimpanzee skin cell line. The cell line has a high saturation density, exhibits colony formation in soft agar, is morphologically altered and produces tumours in nude mice.Only available to customers ordering from within the EU.

General Info

Species

Monkey

Other Collection No.

ATCC CRL 1609

Release Conditions

Restricted - CITES (Convention on the International Trade in Endangered Species of Wild Fauna and Flora) licence required for all orders outside the United Kingdom

Characteristics

Tissue of Origin

Skin

Karyotype

Not specified

Applications

Tumourigenicity and transformation studies

Disease

None Stated

Culture Conditions

Cell Type

Fibroblast-like

Subculture Routine

Split sub-confluent cultures (70-80%) 1:4 to 1:9 i.e. seeding at 1-3x10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Culture Medium

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Growth Mode

Adherent

Additional Info

Depositor

Obtained from ATCC

Country of Origin

United States

GMO Status

Genetically Modified Organism Class 1 (GMO1)

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Proc Natl Acad Sci, USA 1981;78:313

Bibliography

Not specified

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: WES (ECACC 91011612).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.