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UK Health Security Agency

Y1

Y1

Catalogue No.

85062807

Cell Line Name

Y1

Cell Line Description

This cell line is a cloned strain from an adrenal cortex tumour cell line of a male LAF1 mouse. This strain was isolated after alternate passage in tumours in vivo and in cultures in vitro (Proc Natl Acad Sci USA 1962;48:1184-1190). The isolated clones are reported by the original depositor to produce steroid hormones at a high rate (4ug/mg/hr) under ACTH stimulation and cells round up within minutes of ACTH treatment. The hormone produced is reported to be ketosteroid with a hydroxy group at the carbon 20 rather than the usual ketosteroid configuration. However, a laboratory independent to the depositor has since (Dec 2000) tested this steroid producing property using the same method (Cancer Res 1966;26:529) and after 1 hour of stimulation could report no rounding up or change in morphology and a very low cAMP measurement. This laboratory did find that the cells round up when exposed to forskolin (acting through adenylate cyclase) and the original depositor reported morphological changes in response to cholera toxin.

Characteristics

Products

Steroid hormone

Tissue of Origin

Adrenal cortex

Karyotype

2n = 40

Applications

Cloning and hormone studies

Disease

Carcinoma

Culture Conditions

Cell Type

Epithelial

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.05% trypsin/EDTA; 5% CO₂; 37°C.

Culture Medium

Ham's F10 + 2mM Glutamine + 15% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS).

Growth Mode

Adherent

Additional Info

Depositor

Dr W Scheirer, Sandoz Forschungsinstitut GmbH, Vienna

Country of Origin

Austria

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Cancer Res 1966;26:529, Proc Natl Acad Sci USA 1962;48:1184-1190, Compendium of methods for the microbiological examination of foods , 3rd ed. Washington, DC: American Public Health Association; 529-535,1966,

Bibliography

AOAC Official Method 984.35, AOAC International;1995:4.17-4.19, AOAC International; 1995:9.12-9.15, Am J Pathol.1957; 33:631-651, Proc. Soc. Exp. Biol. Med.95, 1957: 304-309, AOAC International 1995;4.23-4.25

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: Y1 (ECACC 85062807).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.