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ZL55

ZL55

Catalogue No.

12050301

Cell Line Name

ZL55

Cell Line Description

ZL55 was derived from an epithelial tumour of the pleural cavity of a 52-year old male, prior to treatment, with a known history of exposure to asbestos.

Characteristics

Receptors

Mesothelial-membrane antigen ME1

Tissue of Origin

Pleural cavity

DNA profile (STR Profile)

Amelogenin: X
CSF1PO: 9,11
D5S818: 10,13
D7S820: 7,11
D13S317: 8
D16S539: 12
TH01: 6
TPOX: 8
vWA: 15,19

Disease

Mesothelioma

Culture Conditions

Cell Type

Epitheloid, Mesenchymal

Subculture Routine

Split subconfluent cultures (70-80%) 1:4 to 1:10 using 0.05% trypsin/EDTA; 5% CO₂; 37°C. Suggested seeding density 1-4x10,000 cells/cm².

Culture Medium

DMEM:HAMS F12 (1:1) + 2mM Glutamine + 15% Foetal Bovine Serum (FBS)

Growth Mode

Adherent

Additional Info

Depositor

Emanuela Felley-Bosco, Laboratory for Molecular Oncology, University Hospital Zurich, Haeldeliweg 4, 8044 Zurich, Switzerland.

Country of Origin

Switzerland

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Schmitter D, Lauber B, Fagg B, Stahel RA.1992 Hematopoietic growth factors secreted by seven human pleural mesothelioma cell lines: interleukin-6 production as a common feature. Int J Cancer.51 (2):296-301. PMID: 1373705.

Bibliography

Frei C, Opitz I, Soltermann A, Fischer B, Moura U, Rehrauer H, Weder W, Stahel R, Felley-Bosco E. 2011 Pleural mesothelioma side populations have a precursor phenotype. Carcinogenesis. 32(9):1324-32. PMID: 21729925.

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: ZL55 (ECACC 12050301).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.