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UK Health Security Agency

MDCK II

MDCK II

Catalogue No.

00062107

Cell Line Name

MDCK II

Cell Line Description

MDCK II - Madin-Darby canine kidney- is a subclone derived from the heterogenous parent line - MDCK (ECACC catalogue no. 85011435) MDCK II cells, which predominate in later passages from MDCK, are reported to display electrical resistance of 100 ohm/cm². This strain is thought to be derived from the distal tubule or collecting duct of the nephron. The cell line can be used as an experimental model to study the generation and maintenance of cell surface polarity in epithelial cells. Mycoplasma eradicated using Boehringer method prior to deposit at ECACC.

Characteristics

Tissue of Origin

Kidney

Karyotype

Dog

Applications

Virus susceptibility : influenza virus, vesicular stomatitis virus and many other viruses.

Disease

None Stated

Culture Conditions

Cell Type

Polarised epithelial

Subculture Routine

Split sub-confluent cultures (70-80%) every 2-3 days; seeding at 1-3 x 10,000cells/cm² using 0.05% tryspin or trypsin/EDTA; 5% CO₂ 37°C,

Culture Medium

MEM + 5% Foetal Bovine Serum (FBS) + 2mM Glutamine.

Growth Mode

Adherent

Additional Info

Depositor

Professor Gerrit van Meer, Dept. of Membrane Enzymology, Centre for Biomembranes and Lipid Enzymology, H.R Kruytgebouw N607, Padualaan 8, 3584 CH Utrecht, The Netherlands.

Country of Origin

Netherlands

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Hansson, G.C.,Simons, K and Van Meer G (1986) EMBO 5: 483-489, Louvard D (1980) PNAS 77; 4132-4136

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: MDCK II (ECACC 00062107).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.