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UK Health Security Agency

WERI

WERI

Catalogue No.

06070602

Cell Line Name

WERI

Cell Line Description

Human retinoblastoma cell line derived from a female Caucasian. The cells are semi-adherent, some neuronal differentiation can occur when subculturing. Tumourigenic in rabbits.

Characteristics

Tissue of Origin

Retina

Morphology

Grape like clusters of round cells

Karyotype

Not specified

Disease

Retinoblastoma

Culture Conditions

Subculture Routine

Split subconfluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-3 x100,000 cells/ml; 5% CO₂; 37°C. Saturation density: 2 x 1000,000 cells/ml. Cells are semi-adherent. Scrape cells from surface; do not use trypsin.

Culture Medium

Iscove's Modified DMEM + 2mM Glutamine + 15% Foetal Bovine Serum (FBS) + 10ug/ml Insulin + 55uM 2-Mercaptoethanol

Growth Mode

Semi-adherent

Additional Info

Depositor

Dr B L Gallie, Ontario Cancer Institiute, 610 University Avenue, Toronto, Canada, M5G 2M9

Country of Origin

Canada

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Corson et al., (2005) KIF14 is a candidate oncogene in the 1q minimal region of genomic gain in multiple cancers. Oncogene 14; 24 (30): 4741-54

Bibliography

Marchong et al., (2004) Minimal 16q genomic loss implicates cadherin-11 in retinoblastoma. Mol Cancer Res; 2(9):495-503. Orlic et al., (2006) Expression analysis of 6p22 genomic gain in retinoblastoma. Genes Chromosomes Cancer; 45(1):72-82

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: WERI (ECACC 06070602).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.