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UK Health Security Agency

KARPAS-45

KARPAS-45

Catalogue No.

06072602

Cell Line Name

KARPAS-45

Cell Line Description

A human T cell leukaemia cell line established from the bone marrow of a 2-year-old boy diagnosed with acute lymphoblastic leukaemia (ALL) prior to therapy in 1972; cells were reported to carry the MLL-AFX and AFX-MLL fusion genes, the original diagnosis was T-ALL.

General Info

Species

Human

Release Conditions

Restricted - commercial organisations are required to complete the 'Cell Line Release Authorisation for Research Use in Commercial Organisations' release conditions form in the supporting documents section.

Characteristics

Tissue of Origin

Bone marrow

Morphology

Spherical

Karyotype

Hypotetraploid

Disease

Lymphoblastic Leukaemia

Culture Conditions

Cell Type

Haematopoietic

Subculture Routine

Maintain cultures between 0.5 - 2 x1000,000 cells/ml; 5% CO₂; 37°C. Doubling time 50-100 hours. Split saturated culture 1:2 to 1:3 every 2-3 days. Spherical cells growing in clusters in suspension.

Culture Medium

RPMI 1640 + 2mM Glutamine + 20% Foetal Bovine Serum (FBS). For slower growth use 10% or 5% FBS.

Growth Mode

Suspension

Additional Info

Depositor

Dr Abraham Karpas, Department of Haematology, University of Cambridge, MRC Centre, Hills Road, Cambridge, UK

Country of Origin

United Kingdom

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Karpas et al., (1977) Leukemia Research 1:35-49, Smith et al., (1973) Lancet: 74-77

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: KARPAS-45 (ECACC 06072602).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.