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UK Health Security Agency

Bob

Bob

Catalogue No.

10021102

Cell Line Name

Bob

Cell Line Description

First spontaneously immortalized prostate cancer cell line to be established from a trans-rectal needle biopsy (TRBP) of a patient with castration-resistant prostate cancer (CRPC). Bob is a novel pre-clinical model for functional studies in CRPC and especially for studying the CRPC "basal" phenotype. This cell line is cultured in serum-free culture medium, a derivative of Bob is also available i.e. SerBob, ECACC catalogue number 10021101, which has been adapted to medium containing serum. SerBob is more differentiated and invasive than Bob. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.

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Characteristics

Receptors

CD44, alpha2 integrin, alpha6 integrin, EGFR, FGFR1, LIFR

Products

10% 3-D sphere formation in Matrigel. Migratory and invasive when compared to benign prostate tumour.

Tissue of Origin

Prostate gland

Morphology

Cobblestone

DNA profile (STR Profile)

Amelogenin: X
CSF1PO: 11,12
D5S818: 13
D7S820: 10,11
D13S317: 9,14
D16S539: 10,12
TH01: 9
TPOX: 8,10
vWA: 17

Karyotype

4n (81 to 94)

Applications

Prostate cancer research including drug testing

Disease

Adenocarcinoma

Culture Conditions

Cell Type

Endothelial

Subculture Routine

Split sub-confluent cultures (70-80%) 1:5 to 1:20 i.e. seeding at 2-4 x 10⁴ cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Cells must be centrifuged at 400 x g for 5 minutes to remove the trypsin at each subculture. Cells are cryopreserved in 45% conditioned media: 45% fresh media: 10% DMSO. At confluence 5-7 x 10⁴ cells/cm² can be expected.

Culture Medium

Keratinocyte-SFM (Invitrogen, catalogue no 37010-022, supplied with prequalified human recombinant epidermal growth factor 1-53 (EGF 1-53), bovine pituitary extract (BPE) and glutamine) + 2ng/ml leukaemia inhibitory factor + 2ng/ml stem cell factor + 100ng/ml cholera toxin + 1ng/ml granulocyte macrophage colony stimulating factor.

Growth Mode

Adherent

Additional Info

Depositor

Drs David Hudson & Sian Rizzo, Institute of Cancer Research, 15 Cotswold Road, Sutton, Surrey, SM2 5NG, UK

Country of Origin

United Kingdom

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Attard G et al., (2009) A novel, spontaneously immortalized, human prostate cancer cell line, Bob, offers a unique model for pre-clinical prostate cancer studies. Prostate 69: 1507-1520 PMID: 19544327.

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: Bob (ECACC 10021102).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.