1.2B4
1.2B4
Cell Line Name
Cell Line Description
1.2B4 is a hybrid cell line formed by the electrofusion of a primary culture of human pancreatic islets with HuP-T3, a human pancreatic carcinoma cell line (ECACC catalogue number 93121055). 1.2B4 has been shown to be tumourigenic when transplanted into a SCID mouse host. The cell line has applications in the study of pancreatic cell biology. 1.2B4 cells provide a method of producing pure insulin secreting cells when stimulated (please see attached protocols for the stimulation of insulin secretion).. This is an alternative to the use of primary tissue in cell transplantation therapies for type 1 diabetes.
Based on evidence from customer feedback we cannot guarantee the cells will produce insulin.
General Info
Species
Human
Links
Cellosaurus: CVCL_2258
Release Conditions
Characteristics
Receptors
Products
Tissue of Origin
DNA profile (STR Profile)
Amelogenin: X,Y
CSF1PO: 10
D5S818: 10,14
D7S820: 12
D13S317: 9,11
D16S539: 10,13
TH01: 9
TPOX: 8,12
vWA: 18
Karyotype
Disease
Culture Conditions
Cell Type
Subculture Routine
Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-4 x 10⁴ cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Population doubling approx 20hrs. At confluence 10⁵ cells/cm² can be expected.
Culture Medium
RPMI-1640 + 2mM Glutamine + 10% FCS
Growth Mode
Additional Info
Depositor
Country of Origin
Hazard Group (ACDP)
Applications
References
Nishimura N et. al., (1993) Newly established human pancreatic carcinoma cell lines and their lectin binding properties Int J Pancreatol 13: 31-41 PMID: 8454916.
Bibliography
Guo-Parke H, McCluskey JT, Kelly C, Hamid M, McClenaghan NH, Flatt PR.2012. Configuration of electrofusion-derived human insulin-secreting cell line as pseudoislets enhances functionality and therapeutic utility. J Endocrinol. 214(3):257-65. PMID: 22685334
Documents
- 10070103_1.2B4_Growth Profile
- Human pancreatic beta cell lines culture and insulin assay protocols-updated July-2014
- Passage numbers explained
- MSDS for nucleic acids
- MSDS For Frozen Cell Culture
- M061 University of Ulster Deposit Release Form for Commercial Organisations
- Important technical information for successful culture of your cells
Images
Available Formats
- Frozen
- DNA-5µg (100ng/µl)
Citation Guidance
If use of this culture results in a scientific publication, it should be cited in the publication as: 1.2B4 (ECACC 10070103).
Biosafety Information
Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.
Further Information
The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.
Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.