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UK Health Security Agency

AC29

AC29

Catalogue No.

10092308

Cell Line Name

AC29

Cell Line Description

Mice (CBA strain female 6-8 weeks) were exposed to crocidiolite asbestos through intraperitoneal injection resulting in tumour development (both ascites and solid tumours). Cultures were established from malignant mesothelial cells obtained from ascites fluid. Cells are tumourigenic in syngeneic imunocompetent mice. Cells grow as adherent monolayers and are epithelial like. This cell line is distributed by ECACC on behalf of CellBank Australia, Children's Medical Research Institute, Westmead, NSW 2145, Australia. All stock supplied is manufactured and Quality Control released at source by Cell Bank Australia. Cell Bank Australia reference number: CBA-0152

Discontinued Message

This product has been discontinued. Partially contaminated. The stock formerly distributed by CBA under the catalogue number CBA-0152 was a mixture of at least two different cell lines from different mouse strains.

Characteristics

Tissue of Origin

Lung

Applications

Investigation of the properties of malignant mesothelioma cells and the development of new diagnostic methods

Disease

Mesothelioma

Culture Conditions

Cell Type

Epithelial-like

Subculture Routine

Optimal split ratio 1:8 - 1:16 (seeding density 0.6 x10⁴ cells/cm²).Cells incubated at 37°C with 5% CO₂. Harvest the cells using 0.05% Trypsin/EDTA at 37°C for 5 min.

Culture Medium

RPMI1640 (with 2mM L-Glutamine+ 25mM HEPES) + 5% FCS

Growth Mode

Adherent

Additional Info

Depositor

Richard Lake - University of Western Australia, Australia

Country of Origin

Australia

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Davis MR, Manning LS, Whitaker D, Garlepp MJ, Robinson BW

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: AC29 (ECACC 10092308).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.