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UK Health Security Agency

RAJI

RAJI

Catalogue No.

85011429

Cell Line Name

RAJI

Cell Line Description

Established in 1963 from Burkitt's lymphoma in an 11 year old male. Growth is in the form of single cells without attachment and as macroscopically visible clumps containing many hundreds of cells. Resistant to VSV. This cell line carries the latent Epstein-Barr Virus (EBV) genome and is positive for EBNA. RAJI is sometimes referred to as a 'non-producer'; the EBV genome carries deletions attributed to preventing the formation of virus particles. Ethnicity: Black.

Characteristics

Tissue of Origin

Lymph

DNA profile (STR Profile)

Amelogenin: X,Y
CSF1PO: 10,12
D5S818: 10,13
D7S820: 10
D13S317: 13
D16S539: 8,11
TH01: 6,7
TPOX: 8,13
vWA: 16,19

Karyotype

2n = 46, diploid

Applications

Resistance and virus studies: VSV

Disease

Burkitt Lymphoma

Culture Conditions

Cell Type

Lymphoblastoid

Subculture Routine

If starting from a frozen ampoule the cryoprotectant should be removed. Add thawed cells to a conical based centrifuge tube e.g. 15ml tube, and then slowly add 4 ml of culture medium to the tube. Take a sample of the cell suspension, e.g. 100µl, to count cells. Centrifuge the cell suspension at low speed i.e. 100 - 150 x g for a maximum of 5 minutes. Remove medium and re-suspend the cell pellet at a density of approximately 5 x 100,000 cells/ml into fresh medium containing 20% serum. Incubate flask at 37°C; 5 - 7% CO₂. Check daily and keep flask in a vertical position until the cells reach the exponential phase of growth. This can take up 3-4 days. Once the culture is established the serum concentration can be reduced to 10%. Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C.

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Growth Mode

Suspension

Additional Info

Depositor

Prof H Harris/Dr R Sutherland, Sir William Dunn School of Pathology, Oxford

Country of Origin

United Kingdom

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Pulvertaft JV 1964 CYTOLOGY OF BURKITT'S TUMOUR (AFRICAN LYMPHOMA).Lancet. 1:238-40 PMID: 14086209; J Nat Cancer Inst 1966;37:547; Trans NY Acad Sci 1966;29:61

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: RAJI (ECACC 85011429).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.