RPMI 6666
RPMI 6666
Cell Line Name
Cell Line Description
Derived from a 29 year old male Caucasian with Hodgkin's disease. One of a large series of permanent lines from leukocytes in the peripheral blood of donors with and without malignancies. Has cell-associated EBV and produces immunoglobulins. This cell line was initially thought to have been derived from Hodgkin lymphoma, but is now known to be an EBV-positive B-lymphoblastoid cell line (Drexler et al., 2003).
General Info
Species
Human
Other Collection No.
Links
Cellosaurus: CVCL_1665
Release Conditions
Characteristics
Products
Tissue of Origin
DNA profile (STR Profile)
Amelogenin: X,Y
CSF1PO: 12,13
D5S818: 11,12
D7S820: 9,10
D13S317: 11
D16S539: 11,12
TH01: 6,9
TPOX: 10,11
vWA: 14,18
Karyotype
Applications
Tumourigenicity studies
Disease
Culture Conditions
Cell Type
Subculture Routine
Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C.
Culture Medium
RPMI 1640 + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).
Growth Mode
Additional Info
Depositor
Country of Origin
Hazard Group (ACDP)
Applications
References
New York State J Med 1966;66:2757, Drexler HG, Dirks WG, Matsuo Y, MacLeod RA. False leukemia-lymphoma cell lines: an update on over 500 cell lines. Leukemia 2003 Feb;17(2):416-26. PMID:12592342
Bibliography
Not specified
Available Formats
- Frozen
- DNA-5µg (100ng/µl)
Citation Guidance
If use of this culture results in a scientific publication, it should be cited in the publication as: RPMI 6666 (ECACC 87081807).
Biosafety Information
Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.
Further Information
The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.
Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.