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UK Health Security Agency

PC-12

PC-12

Catalogue No.

88022401

Cell Line Name

PC-12

Cell Line Description

Derived from a transplantable rat adrenal phaeochromocytoma. Respond reversibly to nerve growth factor by induction of neuronal phenotype. Cells synthesise and store catecholamine, dopamine and NPP. Used in neurobiological and neurochemical studies. When cells are grown with collagen - neuronal fibroblasts; without collagen - spherical clusters. PLEASE NOTE: Growing orders of PC-12 despatched from ECACC are grown without collagen and therefore the cells will be in suspension on receipt.

Characteristics

Products

Catecholamine, dopamine and NPP

Tissue of Origin

Adrenal gland

Karyotype

Not specified

Applications

Neurobiological and neurochemical studies

Disease

None Stated

Culture Conditions

Subculture Routine

Cells grow in small clusters but adhere poorly to plastic. The cells do grow satisfactorily in suspension in untreated flasks. Maintain cultures between 2-5x100,000 cells/ml: 5% CO₂; 37°C. For attachment grow in collagen coated flasks (must be collagen type IV), feed 3 times a week and split cultures 1:3 to 1:6 (i.e. seeding at 2-4 x 10,000 cells / cm²) using 0.05% trypsin/EDTA; 5% CO₂; 37°C. Growing cultures will be supplied in suspension. On receipt, incubate the flask overnight without opening and on introduction to a type IV collagen coated flask the cells should attach. Preparation of collagen solution: Use collagen type IV (Sigma C5533). Add 5mg collagen to 50ml 0.1M glacial acetic acid to obtain a 0.1% collagen solution. Stir at room temperature for 1-3 hours. Store in sterile glass bottles or as pre-coated flasks at 4°C. NB: Growth factor reduced matrigel may also be used. Preparation of flasks: Add sufficient collagen to cover the surface of the required number of tissue culture flasks and leave for at least 6 hours at 37°C. Remove excess fluid and allow flasks to dry by incubating at 37°C, leaving the caps loose. Prior to addition of cells wash flask three times in PBS. Flasks can be purchased from suppliers pre-coated with type IV collagen. Reported doubling time = 92 hours

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Horse Serum + 5% Foetal Bovine Serum (FBS)

Growth Mode

Suspension

Additional Info

Depositor

Obtained from ATCC

Country of Origin

United States

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Methods in Enzymology 1984;1147:207.

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: PC-12 (ECACC 88022401).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.