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UK Health Security Agency

DEM

DEM

Catalogue No.

88052007

Cell Line Name

DEM

Cell Line Description

An Epstein-Barr virus (EBV) transformed lymphoblastoid cell line with Human Leukocyte Antigen (HLA)-typing data available.

General Info

Species

Human

Keywords

Human HLA-Typed Lymphoblastoid Cell Line, German 9007

Release Conditions

Frozen cultures can only be supplied to academic and not for profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.

Characteristics

Tissue of Origin

Blood

Geographical Origin

Germany

Ethnic Origin

Ethnicity Not Specified

IHW Number

9007

Sex

Male

Homozygous

No

Morphology

Spherical

Culture Conditions

Cell Type

Lymphoblastoid

Subculture Routine

When resuscitating from frozen, cells should be seeded at 0.5 x 10⁶ cells/ml in the culture medium. The culture should establish log phase growth after 3-5 days. When growing well dilute every 1-3 days to 3 x 10⁵ cells/ml. Maintain cultures between 3-9 x 10⁵ cells/ml for maximum growth; 5% CO₂; 37°C. Cells form floating aggregates of varying size. Protocol: resuscitation of frozen cells

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS). To help establish a culture the concentration of FBS can be increased to 20%.

Growth Mode

Suspension

Additional Info

GMO Status

Genetically Modified Organism Class 1 (GMO1)

Hazard Group (ACDP)

2

HLA Profile

*Information as provided at time of deposit of the cell line into the ECACC HLA-Typed Collection.

Serological Profile: HLA Class I & Class 2 Antigens

HLA A

2

HLA A ief

2.2

HLA C

6

HLA B

57

HLA B ief

57.2

Bw4/bw6

4

HLA DR

4 16

HLA DR51

51

HLA DR53

53

HLA DQ

5 8

HLA DP

3 4

DNA Profile: HLA Class I & Class 2 Antigens

A*

201

Cw*

602

B*

5701

HLA DRA*

101

HLA DRB1*

0401 1602

HLA DRB4*

101

HLA DRB5*

2

HLA DRB6*

202

HLA DQA1*

0102 03

HLA DQB1*

0502 0302

HLA DPA1*

1

HLA DPB1*

0301 0401

Non-HLA Genes (Genes within the HLA Region)

BF

S

C4A

A6

C4B

B1

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: DEM (ECACC 88052007)

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.