C-4-II
C-4-II
Cell Line Name
Cell Line Description
From a cervical carcinoma of a 41 year old Caucasian female. Cells form spreading predominantly monolayered, epithelial colonies with hemicysts which are characteristic of transporting epithelia and respond to crowding by separation and shedding. These cells have retained properties of basal cell differentiation in long term culture and exhibit G6PD type B. C-4 II cells have been reported to contain human papilloma virus 18 (HPV-18) DNA sequences and to express HPV-18 RNA.
General Info
Species
Human
Other Collection No.
Links
Cellosaurus: CVCL_1095
Release Conditions
Characteristics
Tissue of Origin
DNA profile (STR Profile)
Amelogenin: X
CSF1PO: 12
D5S818: 9,11
D7S820: 10,11
D13S317: 11,12
D16S539: 11
TH01: 9,9.3
TPOX: 10,11
vWA: 14
Karyotype
Applications
Differentiation studies
Disease
Culture Conditions
Cell Type
Subculture Routine
Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C.
Culture Medium
Waymouth's MB 752/1 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Growth Mode
Additional Info
Depositor
Country of Origin
Hazard Group (ACDP)
Applications
References
J Nat Cancer Inst 1962;28:605; J Nat Cancer Inst 1969;43:151; J Nat Cancer Inst 1969;43:175
Bibliography
Not specified
Available Formats
- Frozen
- DNA-5µg (100ng/µl)
Citation Guidance
If use of this culture results in a scientific publication, it should be cited in the publication as: C-4-II (ECACC 88102701).
Biosafety Information
Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.
Further Information
The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.
Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.