ARH 77
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ARH 77
Cell Line Name
Cell Line Description
Derived from a 33 year old female with plasma cell leukaemia. The cells synthesise IgG1 (kappa-light chain) and are EBNA positive.Although the cells are reported to originate from a patient with a plasma cell leukaemia (Cancer Res 1978;38:2508), it has now been shown that this is in fact an EBV transformed B lymphoblastoid cell line (Leukaemia 1999;13:1601-1607).
General Info
Species
Human
Other Collection No.
Links
Cellosaurus: CVCL_1072
Release Conditions
Characteristics
Products
Tissue of Origin
DNA profile (STR Profile)
Amelogenin: X
CSF1PO: 6,10
D5S818: 10,13
D7S820: 7,12
D13S317: 11,13
D16S539: 9,13
TH01: 8,9.3
TPOX: 8
vWA: 17
Karyotype
Disease
Culture Conditions
Cell Type
Subculture Routine
Maintain cultures between 1-4x100,000 cells/ml; 5% CO₂; 37°C. Maximum cell density 1-2x1,000,000 cells/ml.
Culture Medium
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Growth Mode
Additional Info
Depositor
Country of Origin
Hazard Group (ACDP)
Applications
References
Cancer Res 1978;38:2508
Bibliography
Cancer Res 1978;38:2508 Proc Nat Acad Sci. USA 1983;80:2026, Cancer Res. 38:2508-2513,1978 PubMed: 78212770,Proc. Natl. Acad. Sci. USA 80:2026-2030, 1983 PubMed: 83169813,Eur.J. Immunol. 12:641-648,1982 PubMed:83053493,Pro.Am.Assoc.Cancer Res.19:211,1978
Available Formats
- Frozen
- DNA-5µg (100ng/µl)
Citation Guidance
If use of this culture results in a scientific publication, it should be cited in the publication as: ARH 77 (ECACC 88121201).
Biosafety Information
Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.
Further Information
The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.
Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.