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UK Health Security Agency

MEG-01

MEG-01

Catalogue No.

94012401

Cell Line Name

MEG-01

Cell Line Description

Derived from the bone marrow of a 55 year old male with chronic myelogenous leukaemia. All karyotypes show the Philadelphia chromosome. Platelet GP IIb/IIIa antigen is expressed on more than 92% of cells. Cytoplasmic expression of GP Ib antigen was not detected on the surface of cells but was present in the cytoplasm 20% of cells. BA-1 antigen is expressed in almost all cells. FVIII-related antigen was not detected on the surface cells but was present in cytoplasm.

Characteristics

Products

Immunoglobulin G (IgG)-Fc in 12% of cells; C3' in 53% of cells

Tissue of Origin

Bone marrow

DNA profile (STR Profile)

Amelogenin: X,Y
CSF1PO: 10
D5S818: 13
D7S820: 11
D13S317: 8
D16S539: 9
TH01: 7
TPOX: 8,11
vWA: 16

Karyotype

Modal no. 56-58, hyperdiploid

Applications

Studies of physiological mechanisms governing the differentiation of human megakaryocyte and platelet release; the study of the biochemistry (and differentiation) of erythroid and myeloid cells.

Disease

Myelogenous Leukaemia

Culture Conditions

Cell Type

Myoblast-like

Subculture Routine

Subcultures are prepared by scraping the adherent cells into the medium. From the resulting cell suspension cells should be seeded into fresh medium at 2-4 x 10⁵ cells/ml. Doubling time is 36-48 hours. 5% CO₂; 37°C

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Growth Mode

The cell line grows as a mixture of adherent and suspension cells

Additional Info

Depositor

Dr M Takeuchi, Institute for Fermentation, Osaka

Country of Origin

Japan

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Ogura M, Morishima Y, Ohno R, Kato Y, Hirabayashi N, Nagura H, Saito H.1985 Establishment of a novel human megakaryoblastic leukemia cell line, MEG-01, with positive Philadelphia chromosome Blood 66(6):1384-92 PMID: 2998511

Bibliography

Takeuchi K, Ogura M, Saito H, Satoh M, Takeuchi M 1991 Production of platelet-like particles by a human megakaryoblastic leukemia cell line (MEG-01).Exp Cell Res. 193(1):223-6.PMID: 1995298

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: MEG-01 (ECACC 94012401).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.