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UK Health Security Agency

DH82

DH82

Catalogue No.

94062922

Cell Line Name

DH82

Cell Line Description

Derived from a ten year old male golden retriever with malignant histiocytosis. The cells have a macrophage-like morphology and are able to phagocytose latex particles. They are positive for Fc-gamma receptors and are negative for Fc-mu and C3b receptors. DH82 cells do not produce IL-1.

Characteristics

Receptors

FC-gamma receptors

Tissue of Origin

Not specified

Karyotype

Not specified

Disease

Sarcoma

Culture Conditions

Cell Type

Macrophage

Subculture Routine

Cells are semi-adherent, i.e. some cells grows in suspension, whilst most attach to the surface and may flatten. Attached cells should be removed with 0.05% Trypsin/EDTA. Split sub-confluent cultures (70-80%) i.e. seeding at 2-4x10,000 cells/cm².

 

Recommended growth conditions 5% CO2; 37°C.

 

Note: Do not agitate the cells by hitting or shaking flask whilst waiting for cells to detach via trypsinisation to avoid cells clumping together.

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 15% Foetal Bovine Serum (FBS) (Heat Inactivated).

Growth Mode

Suspension

Additional Info

Depositor

Obtained from ATCC

Country of Origin

United States

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Wellman ML, Krakowka S, Jacobs RM, Kociba GJ 1988 A macrophage-monocyte cell line from a dog with malignant histiocytosis. In Vitro Cell Dev Biol.24(3):223-9. PMID: 3350786.

Bibliography

Gröne A, Fonfara S, Baumgärtner W. 2002 Cell type-dependent cytokine expression after canine distemper virus infection. Viral Immunol. 15(3):493-505. PMID: 12479398.

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: DH82 (ECACC 94062922).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.