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UK Health Security Agency

SK-PN-DW

SK-PN-DW

Catalogue No.

94092301

Cell Line Name

SK-PN-DW

Cell Line Description

SK-PN-DW has been derived from a male patient with a primitive neuroectodermal tumour that expresses cholecystokinin (cck) RNA and synthesises substantial amounts of pro-cck. SK-PN-DW is able to process the pro-hormone, producing immunoreactive cck-like material plus a peptide that immunochemically and chromatographically resembles intact cck c-terminal extension peptide. Cells have a tendency to float.

Characteristics

Receptors

Vimentin, P53, anti-MIC2

Products

Pro-cholecystokinin (Pro-cck)

Tissue of Origin

Neural

Morphology

Polygonal

DNA profile (STR Profile)

Amelogenin: X
CSF1PO: 12
D5S818: 13,14
D7S820: 8,14
D13S317: 9
D16S539: 12
TH01: 6
TPOX: 8,9
vWA: 16

Karyotype

82XXY

Applications

Tumour lines should prove useful in understanding the regulation of the cck gene in human neuronal cells and will also provide an `in vitro' system for investigation of the post translational processing of the cck preprohormone.

Disease

Neuroectodermal Tumour

Culture Conditions

Cell Type

Epithelial-like

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Culture Medium

DMEM or EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Growth Mode

Semi-adherent

Additional Info

Depositor

Mrs C Helson, St Agnes Hospital, 305 North St, White Plains, New York

Country of Origin

United States

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

J Clin Endocrin and Metab 1989;69(2):411; Cancer Genet Cytogenet 1987;24:75

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: SK-PN-DW (ECACC 94092301).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.