SK-N-AS
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SK-N-AS
Cell Line Name
Cell Line Description
Derived from a female patient with neuroblastoma from the metastasis of the bone marrow. Tumourigenic in nude mice.
General Info
Species
Human
Other Collection No.
Links
Cellosaurus: CVCL_1700
Release Conditions
Characteristics
Receptors
Tissue of Origin
Morphology
DNA profile (STR Profile)
Amelogenin: X
CSF1PO: 10,12
D5S818: 11,12
D7S820: 11,13
D13S317: 9
D16S539: 14
TH01: 9.3
TPOX: 11,12
vWA: 16,17
Karyotype
Applications
Neuroblastoma differentiation studies
Disease
Culture Conditions
Cell Type
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 2-4 x 10,000 cells/cm² using 0.05% trypsin/EDTA; 5% CO₂; 37°C.
Culture Medium
DMEM or EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Growth Mode
Additional Info
Depositor
Country of Origin
Hazard Group (ACDP)
Applications
References
Cell Growth and Differentiation 1991;2:487; Cancer Res 1995;55:2380; Int J Oncology 1945;5:1037; J Clin Investigation Inc 1989;84:829
Available Formats
- Frozen
- DNA-5µg (100ng/µl)
Citation Guidance
If use of this culture results in a scientific publication, it should be cited in the publication as: SK-N-AS (ECACC 94092302).
Biosafety Information
Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.
Further Information
The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.
Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.