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UK Health Security Agency

BE(2)-M17

BE(2)-M17

Catalogue No.

95011816

Cell Line Name

BE(2)-M17

Cell Line Description

The cell line BE(2)-M17 was isolated from SK-N-BE(2) (ECACC catalogue no. 95011815) by limiting dilution. The cells show a population doubling time of 20-24 hours, grow in multi-layers but start to aggregate and float as the culture ages. The neuroblastic cells express properties of neuroadrenergic neurones and multiple, moderately long cell processes. BE(2)-M17 showed tyrosine hydroxylase and dopamine-ß-hydroxylase activity and specific uptake of norepinephrine (noradrenalin). Expression of intermediate filaments was detected as well as neuron-specific enolase and moderate expression of vimentin. BE(2)-M17 are tumorigenic in nude mice.

Characteristics

Tissue of Origin

Neural

Morphology

Fusiform

DNA profile (STR Profile)

Amelogenin: X
CSF1PO: 10
D5S818: 12
D7S820: 9,10
D13S317: 11
D16S539: 11
TH01: 6
TPOX: 8,11
vWA: 18

Karyotype

Not specified

Applications

Study of neuroblastoma

Disease

Neuroblastoma

Culture Conditions

Cell Type

Fibroblast-like

Subculture Routine

Split sub-confluent cultures (70-80%) 1:8 to 1:20 i.e. seeding at 1 - 4x10,000 cells/cm² using 0.1% trypsin; 5% CO₂; 37°C. Seed at 3-4 x 10,000 cells /cm² on resuscitation from frozen.

Culture Medium

EMEM (EBSS):Ham's F12 (1:1) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 15% Foetal Bovine Serum (FBS) (Heat Inactivated).

Growth Mode

Adherent

Additional Info

Depositor

Dr R A Ross and Dr BA Spengler, Department of Biological Sciences, Fordham University, New York

Country of Origin

United States

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Cancer Res 1989;49:219; Cancer Res 1993;53:4978

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: BE(2)-M17 (ECACC 95011816).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.