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UK Health Security Agency

SUP-T1

SUP-T1

Catalogue No.

95013123

Cell Line Name

SUP-T1

Cell Line Description

Derived from a Malignant pleural effusion taken from an eight year old caucasian male with T cell lymphoblastic lymphoma. These cells are E-rosette, Leu 5, OKT9 and CALLA negative. They also express beta2M, Leu 1, 2, 3a, 4, 6, 9 and OKT10 antigens and are CD4 positive. These cells are negative for Tac, HLA-DR and surface immunoglobulin.Has chromosomal translocations involving the genes for the alpha and beta subunits of TCR thus preventing surface expression of the endogenous TCR complex.

Characteristics

Receptors

CD4

Tissue of Origin

Blood

Karyotype

92XXXY, -2, -2, -9, -9, 2inv(2*)(p21q11), +2inv(2), del(2*)(Q21), 2del(4)(q31q35), 2del (5)(p13p14), 2del(6)(q23q27), inv(14)(q11q32), t(7;9)(q34,q34), +der(9)t(7;9). Undergone Tetraploidisation

Applications

Cells have been used for growth of HIV-1

Disease

Lymphoma

Culture Conditions

Cell Type

Lymphoblastoid

Subculture Routine

Maintain cultures between 2x100,000 to 2x1,000,000 cells/ml 5% CO₂; 37°C.

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Growth Mode

Suspension

Additional Info

Depositor

Obtained from ATCC

Country of Origin

United States

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Cancer Res 1984;44:5657; Cell 1985;43:705; ibid 1986;50:107; Science 1984;226:1445; ibid 1986;232:1123; Science 1986; 232: 1123

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: SUP-T1 (ECACC 95013123).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.