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Ampli-GPE

Ampli-GPE

Catalogue No.

95050229

Cell Line Name

Ampli-GPE

Cell Line Description

The retrovirus packaging Ampli-GPE (clone A57) cells were obtained from NIH3T3 fibroblasts (ECACC catalogue no. 93061524) being co-transfected with two kinds of recombinant chimeric proviral genes in the BMG Neo vector. To reduce the homology between proviral DNA in the packaging cell and the retroviral vector, the gag-pol and env genes of Moloney murine leukaemia virus were separated onto two different plasmids in which the 5' long terminal repeat (LTR) and the 3' LTR had been replaced by the mouse metallothionein I promoter or the rabbit ß-globin gene. This expression and amplification system produces a viral titre high enough for gene transfer to somatic cells (up to 5x1,000,000 CFU/ml) and avoids the generation of replication-competent viruses.

Characteristics

Tissue of Origin

Embryo

Karyotype

Not specified

Applications

Retrovirus packaging, gene transfer assays

Disease

None Stated

Culture Conditions

Cell Type

Fibroblastic

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-5x10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Culture Medium

DMEM + 2mM Glutamine + 250µg/ml G418 + 10% Foetal Bovine Serum (FBS).

Growth Mode

Adherent

Additional Info

Depositor

Dr Y Takahara, Central Research Laboratories, Ajinomoto Co., Kawasaki

Country of Origin

Japan

GMO Status

Genetically Modified Organism Class 1 (GMO1)

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

J Virol 1992;66:3725

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: Ampli-GPE (ECACC 95050229).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.