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DMS 454

DMS 454

Catalogue No.

95062832

Cell Line Name

DMS 454

Cell Line Description

The small cell carcinoma DMS 454 was isolated in 1981 from lung tissue of a male patient at time of autopsy. The cells contain dense-core vesicles and secrete calcitonin.

Characteristics

Products

Calcitonin

Tissue of Origin

Lung

Morphology

Polygonal

DNA profile (STR Profile)

Amelogenin: X,Y
CSF1PO: 11,12
D5S818: 12
D7S820: 8
D13S317: 11,12
D16S539: 11,13
TH01: 6,9
TPOX: 8
vWA: 18

Karyotype

Not specified

Applications

Human lung tumour model, hormone secretion

Disease

Small Cell Cancer

Culture Conditions

Cell Type

Epithelial-like

Subculture Routine

Cells grow as small dense islands and will not form a monolayer, maximum confluency is approximately 60%. Split cells seeding at 2-4 x 10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA.

 

Recommended growth conditions 5% CO2; 37°C.

Culture Medium

Waymouth's MB 752/1 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) (heat-inactivated).

Growth Mode

Adherent

Additional Info

Depositor

Prof O S Pettengill/Dr G D Sorenson, Dartmouth Medical School, Lebanon

Country of Origin

United Kingdom

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Lung Cancer 1984:203 (Aisner J (ed). New York)

Bibliography

Barretina J, et al., 2012 The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity. Nature. 483(7391):603-7. PMID: 22460905.

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: DMS 454 (ECACC 95062832).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.