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CLC

CLC

Catalogue No.

95070628

Cell Line Name

CLC

Cell Line Description

The cell line CLC (Carp Leukocyte Culture) was established from peripheral blood mononuclear cells of a normal 2 year old female common carp, Cyprinus carpio. They possess phagocytic activity and are reported to be stimulated by TNF, gamma-interferon and LPS therefore resembling characteristic features of monocytes / macrophages. CLC replicate fish viruses e.g. IPNV, PFR, SVCV and VHSV.

Characteristics

Tissue of Origin

Blood

Karyotype

2n = 104

Applications

Immunological research, fish virology, fish cell physiology

Disease

None Stated

Culture Conditions

Cell Type

Epithelial-like

Subculture Routine

Spilt confluent cultures 1:3 using 0.05% trypsin or trypsin/EDTA; 25-28°C. Fish cell lines detach easily during transit if the culture is too young. After resuscitation split cells 1:3 and culture for at least one week with 1-2 media changes before shipping.

Culture Medium

EMEM (EBSS) or TC-199 + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Growth Mode

Adherent

Additional Info

Depositor

Prof W Ahne, Institute of Zoology, Fishery Biology and Fish Diseases, University of Munich

Country of Origin

Germany

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Dev Comp Immunol 1990;14:255

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: CLC (ECACC 95070628).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.