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PE/CA-PJ34 (clone C12)

PE/CA-PJ34 (clone C12)

Catalogue No.

97062513

Cell Line Name

PE/CA-PJ34 (clone C12)

Cell Line Description

The human basaloid squamous cell line PE/CA-PJ34 (clone 12) has been established from the oral cavity of a 60 year old male patient in December 1996. The cells, that are also known as Plaepi 34, express cytokeratin.

Characteristics

Tissue of Origin

Oral cavity

DNA profile (STR Profile)

Amelogenin: X
CSF1PO: 10
D5S818: 10,12
D7S820: 9,10
D13S317: 12
D16S539: 9,13
TH01: 6
TPOX: 8
vWA: 18

Karyotype

Not specified

Applications

Study of human squamous carcinoma

Disease

Squamous Cell Carcinoma

Culture Conditions

Cell Type

Epithelial

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:8 i.e. seeding a 1-4 x 10,000 cells/cm using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Culture Medium

IDMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Growth Mode

Adherent

Additional Info

Depositor

Drs H Kosmehl, A Berndt, Friedrich Schiller University, Jena, Germany

Country of Origin

Germany

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Invasion Metastasis 1997;17:251

Bibliography

Barretina J, et al., 2012 The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity. Nature. 483(7391):603-7. PMID: 22460905.

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: PE/CA-PJ34 (clone C12) (ECACC 97062513).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.