New cell lines

OX-133

October 2021

OX-133, a monoclonal antibody recognizes protein-bound N-ethylmaleimide (NEM) bound to cysteine residues in proteins. OX-133 detects NEM-modified proteins on the cell surface and can be used as an affinity matrix to purify NEM-modified proteins from cell lysates. It does not cross react with any other alkylating agent, making it a highly selective reagent for the purification of NEM-labelled proteins and potentially peptides for mass spectrometry-based analysis.

Catalogue number	Cell line name	Keywords
15090201	OX-133	Antibody against N-ethyl maleimide (NEM) cysteine

 

N14A and N15A

February 2021

Pseudomyxoma peritonei (PMP) is a rare tumour of appendiceal origin which is treated primarily with cytoreductive surgery, however the morbidity is high. PMP is considered resistant to most chemotherapies and its molecular biology little understudied. Currently there is no platform for pre-clinical drug testing against this neoplasm. The development and availability of two immortalised cell lines (N14A and N15A) provides a resource fit for pre-clinical anti-tumour drug testing and oncogene activity in Pseudomyoma peritonei (PMP) tumour biology. 

Catalogue number	Cell line name	Keywords
16110902	N14A	Pseudomyxoma peritonei, PMP, appendicitus, appendiceal origin, appendix
16110903	N15A	Pseudomyxoma peritonei, PMP, appendicitus, appendiceal origin, appendix

HeLa mCherry-Histone H2B EGFP-Alpha Tubulin

January 2020

When cells divide the genetic material, DNA, is duplicated and then shared equally between the two daughter cells in the form of chromosomes. To enable this process a molecular chromosome division mechanism, called the mitotic spindle develops inside dividing cells. The mitotic spindle is built out of minute strings called microtubules. The chromosomes attach to these strings so that they can be pulled into the daughter cells by the mitotic spindle. If anything goes wrong during this process, chromosomes may be lost or gained. If this happens, further cell divisions will be impaired, leading to cell death or initiation events in cancer development. The unequal segregation of the genetic material, a condition called aneuploidy, is considered a hallmark as well as a driving force of cancer. To investigate the mechanisms of cell division in detail in living cells, the University of Liverpool, Cancer Research Centre have developed two HeLa cell lines stably expressing EGFP-tagged histone H2B and mCherry-tagged histone H2B -EGFP-tagged alpha-tubulin. These have been generated in which the genetic material and the microtubule strings have been made fluorescent so that they can be observed while cells divide under the microscope. This allows for analysis of the cell division process as it occurs and can aid in highlighting which processes in cells can go wrong during mitosis.

Catalogue number	Cell line name	Keywords
17100525	HeLa mCherry-Histone H2B EGFP-Alpha Tubulin	EGFP, Human Histone H2B, mCherry, Alpha Tubulin, fusion protein, H2B-mCherry, EGFP-Alpha Tubulin

 

HT29 Derivatives

December 2020

The definition of the biological characteristics for resistance to chemotherapeutic treatments in tumour cells is important for developing new treatment strategies. Human colon carcinomas are heterogeneous at the molecular and cellular levels and pose a major challenge in defining the characteristics which lead to resistance to chemotherapy. Distinct sub-populations of mucus-secreting cells have been derived from the colon cancer cell line HT29 after long-term treatment with the anti-cancer drugs, 5-fluorouracil (5FU) and methotrexate (MTX) by the research group led by Thécla Lesuffleur and Alain Zweibaum (Inserm). Mucins are increasingly implicated as playing a role in carcinogenesis and their expression have been studied in drug-resistant subpopulations of the HT29 colon carcinoma cell line. These subpopulations and clones display different patterns of mucin expression in addition to differing resistance to MTX and 5FU and provide useful in vitro models to study the potential role of mucins or other biological markers in drug resistance pathways. 

Catalogue number	Cell line name	Keywords
15121704	HT29-5FU resistant (1 microMolar)	HT29-5FU, 5-Fluorouracil-resistance, goblet cells, gastric mucins. dome-forming cells, 5FU
15121705	HT29-MTX resistant (1 microMolar)	HT29-MTX, methotrexate-resistance, goblet cells, gastric mucins, MTX
15121709	HT29-5FU resistant (10 microMolar)	HT29-5FU, 5-Fluorouracil-resistance, goblet cells, gastric mucins, 5FU, 5-fluorouracil, 5-FU
15121710	HT29-MTX resistant (100 microMolar)	Colon carcinoma, drug resistance, methotrexate, HT29-MTX, methotrexate-resistance, goblet cells, gastric mucins
15121711	HT29-MTX resistant (10 microMolar)	HT29-MTX, methotrexate-resistance, goblet cells, gastric mucins

 

Rev-Erb alpha (NR1D1)^NEW 

November 2020

At the molecular level the circadian clock is conserved in all cell types and is driven by a range of transcriptional factors and loops (involving BMAL1, CLOCK and NPAS2) and rhythmic negative feedback which include Cry1/2, Per1/2 and the nuclear receptors REV-ERBα and REV-ERBβ (also known as NR1D1 and NR1D2). These nuclear receptors act as rhythmic repressors to limit inflammatory activity (from inflammatory triggers LPS or cytokine challenges). REV-ERBα within the airway epithelial cells, and the sentinel macrophages, plays a critical role in pulmonary inflammation, and that REV-ERBα serves as a signalling relay for 2-way communication between the core circadian clock and elaboration of the inflammatory reaction. REV-ERBα is dominant over REV-ERBβ, but there is some redundancy between the 2 paralogs. The monoclonal antibody produced (REV-ERBα (NR1D1)) specifically detects REV-ERBα but not its paralog REV-ERBβ (NR1D2).

 

Catalogue number	Cell line name	Keywords
19061801	Rev-Erb alpha (NR1D1)	Nuclear Receptor subfamily 1, group D member 1, (NR1D1), Hybridoma (6F05-2), mouse mAb

 

KT15^NEW

November 2020

Hybridoma (clone KT15) was established by the fusion of mouse myeloma cells (NS0) with spleen cells from Sprague Dawley rats immunised with mouse T cell clone, C6, H-2KK restricted antigen specific cytotoxic lymphocytes (CTL). It is a monoclonal producing antibody which reacts with a non-polymorphic epitope on the mouse CD8 alpha chain (i.e. mouse Ly2.1 and Ly2.2 cells). It produces monoclonal antibodies with subclass Rat IgG2aλ.

Catalogue number	Cell line name	Keywords
19031501	KT15	T cell clone, C6, H-2KK restricted antigen specific CTL, monoclonal antibody, mouse

 

Aag2-AF5

June 2020 

Insect lines have been used in the propagation of viruses and for the elucidation of molecular mechanisms of cellular responses to viral infection. Viruses transmitted by arthropod vectors to vertebrate hosts are known as arboviruses, and they are spread by vectors, including mosquitoes, ticks, midges, and sandflies. Arboviruses most commonly belong to the Bunyaviridae, Togaviridae, Flaviviridae, and Reoviridae families. In many instances they greatly impact human and animal health. Mosquitoes of the Aedes genus transmit the human-pathogenic chikungunya (CHIKV), dengue (DENV), and Zika (ZIKV) viruses. Aag2-AF5 is a single cell clone derived from the Aag2 cell line. Aag2-AF5 is immunocompetent for RNAi (siRNA and piRNA pathways) and NF-κB signalling.

 

Catalogue number	Cell line name	Keywords
19022601	Aag2-AF5	Aedes aegypti, clonal cell line

 

Aag2-AF319^NEW

June 2020

Arboviruses that most commonly belong to the Bunyaviridae, Togaviridae, Flaviviridae, and Reoviridae families are transmitted by insect vectors, including mosquitoes, ticks, midges, and sandflies. The major antiviral response in mosquitoes is a sequence-specific RNA degredation mechanism called RNA interference (RNAi). Several pathways that differ in their induction including effector proteins, and small RNA molecules: small interfering RNA (siRNA), microRNA (miRNA), and PIWI-interacting RNA (piRNA) may also be involved. The exogenous siRNA (exo-siRNA) pathway is triggered through dicer 2 (Dcr2) recognition of virus double-stranded RNA (dsRNA), which is processed into 21-nucleotide (nt)-long virus-specific siRNAs (vsiRNA) that are unwound and loaded into Argonaute 2 (Ago2) in the multiprotein RNA-induced silencing complex (RISC). It is assumed that the complementary strand of the vsiRNA duplex is degraded, while the remaining strand guides Ago2 to complementary viral RNA, followed by cleavage and degradation of the target RNA. Arbovirus-specific vsiRNAs have been reported for a variety of arbovirus-infected mosquitoes. In particular it has been shown to play a key role in the antiviral response, as its knockdown enhances virus replication. Aag2-AF319 is a clonal homozygous cell line containing a Dcr2 knockout mutation and should facilitate the characterisation of other non-canonical mechanisms in antiviral response.

 

Catalogue number	Cell line name	Keywords
19022602	Aag2-AF319	Aedes aegypti-derived clonal cell line, Dcr2 knockout, RNAi deficiency

 

 

SG01

Cell line SG01 is a novel subglottic epithelial cell line which provides a unique resource for researchers to investigate subglottic diseases, such as subglottic stenosis. The subglottis is a crucial site for upper airway infection, inflammation and malignancy (specifically laryngeal cancer). It also has a crucial relationship with the lower airway in terms of infection. Previous models have included locations such as the nose or lung, however this is unlikely to fully recapitulate the subglottic airway in humans, which is a distinct respiratory niche. SG01 is an immortalised cell line that can differentiate at the air-liquid interface (ALI) in culture and demonstrates morphology of normal epithelium and cytokeratins of normal epithelium and relevant ion channel expression associated with transepithelial resistance.

 

Catalogue number	Cell line name	Keywords
17100230	SG01	Subglottic stenosis, cancer, inflammation, immortalised, cell line

 

Wilms’ Tumor 17.94

Genetic and epigenetic defects in the developing kidney that disrupt the normal differentiation pathway of the metanephric blastema into epithelial and stromal components in the maturing kidney gives rise to ‘Wilms’ Tumor’. These paediatric renal tumours occur at approximately 1 in 10,000 children. Most Wilms’ Tumors retain some differentiation potential, exhibiting so-called “triphasic” histology in which undifferentiated blastema can be observed, together with differentiated epithelial and stromal cells. Considerable advances in understanding the molecular pathogenesis of Wilms’ Tumor has been made, however, the cell biology of this tumour type is less well understood due to the small number of established Wilms’ Tumor cell lines. The establishment of a new Wilms’ Tumor cell line, Wilms’ Tumor 17.94, that has features expected of transformed metanephric blastemal cells, including some apparent potential for in vitro differentiation provides another model to study this disease.

 

Catalogue number	Cell line name	Keywords
17100502	Wilms’ Tumor 17.94	Wilms’ Tumor, cell line, anaplastic

 

HeLa EGFP-Histone H2B

Dynamic protein phosphorylation is mediated by a conserved cohort of protein kinases controlling the profound changes in cellular organization required for mitosis and cytokinesis. Many protein kinases are activated by a conserved regulatory step involving T-loop phosphorylation. Although there is considerable focus on kinase activator proteins, the importance of specific T-loop phosphatases reversing kinase activation has been underappreciated.  The protein phosphatase 6 (PP6) holoenzyme is the major T-loop phosphatase for Aurora A, an essential mitotic kinase. Loss of PP6 function by depletion of catalytic or regulatory subunits interferes with spindle formation and chromosome alignment due to increased Aurora A activity.

Two HeLa cell lines have been created to determine if PP6 catalytic activity is required for normal nuclear morphology, mitosis and cytokinesis.  These cell lines, one stably expressing EGFP-tagged histone H2B the other expressing mCherry-tagged histone H2B and EGFP-tagged alpha-tubulin, have been used in the live-cell imaging of cells revealing that PPP6C-depletion in cells does not form a bipolar spindle with normal kinetics and results in failure to efficiently align chromosomes at the metaphase plate.

 

Catalogue number	Cell line name	Keywords
17100529	HeLa EGFP-Histone H2B	PP6 catalytic activity, EGFP, EGFP-tagged histone H2B

 

HEK293

The HEK293 GFP-WIPI1 and HEK293 GFP-WIPI2 cell lines have been developed to study autophagy by light microscopy. These stable cell line expressing GFP-tagged human WIPI1 and WIPI2 proteins are in a background of the HEK293A cell line. They are useful in studying the function of the phosphatidylinositol 3-phosphate [PtdIns(3)P] effector WIPI proteins by identifying WIPI1 and WIPI2 interacting proteins They are also useful for high throughput microscopy screens. The research areas where the function of these proteins can be studied include: autophagy and cell stress, apoptosis and programmed cell death, cancer, cell signalling & signal transduction, cellular transport, drug discovery & development, metabolism.

 

Catalogue number	Cell line name	Keywords
17100531	HEK293-A mRFP-ATG9	Autophagy, Cancer, Cell Signalling, Signal Transduction, Cellular Transport, Drug Discovery & Development, Metabolism
17100532	HEK293 GFP-WIPI2	Cancer, neurodegeneration, autophagy
17100533	HEK293 GFP-WIPI1	Cancer, neurodegeneration, autophagy

 

High grade serous ovarian cancer

High grade serous ovarian cancer (HGSOC) is the most frequent histological ovarian cancer subtype. While most patients obtain a pathological complete response after first-line of chemotherapy, most of them will relapse and develop resistant fatal disease. Overall survival for women with HGSOC has not improved in the last two decades and five-year survival is less than 30%. The availability of high grade serous ovarian cancer cell line models is limited with the existing models often failing to recapitulate disease phenotypes. Three new spontaneously immortalized continuous cell lines – CIOV1, CIOV2, and CIOV3 – derived from different patients with high grade serous ovarian cancer were generated and characterised using tagged amplicon sequencing, immunohistochemistry /immunocytochemistry and shallow whole genome sequencing. Each cell line retained the genomic and pathologic characteristics of the parental tumour. All three cell lines were thoroughly characterised including their chemosensitivity to standard therapeutic drugs making them valuable models to study acquired and primary chemoresistance of HGSOC.     

 

Catalogue number	Cell line name	Keywords
17100534	CIOV1	Ovarian cancer, HGSOC
	CIOV2	*Not yet available. Coming soon to ECACC*
17100536	CIOV3	High grade serous ovarian cancer, TP53 mutation, BRCA1 and BRCA2 mutations, PTEN, RB1

 

 

EBiSC and CHDI

January 2020

The European Bank for induced Pluripotent Stem Cells (EBiSC) and CHDI Foundation have collaborated with Censo Biotechnologies to generate a cohort of 45 iPSC lines derived from Huntington’s disease (HD) gene-expansion carriers (HDGECs) and associated controls.

These new lines will be used to further investigate the mechanisms of HD progression and for the development of novel therapeutics and will be widely available to any interested researchers. 24 of these cell lines are currently available on our website with more coming soon. 

You can view our CHDI cell lines here

 

NIMP-R14 

January 2020 

The NIMP-R14 cell line is a rat hybridoma, producing a monoclonal antibody IgG2b which detects neutrophils. 

Catalogue number	Cell line name	Keywords
18071701	NIMP-R14	Hybridoma, BALB/c mouse neutrophils, Anti-RTN4IP1 antibodies, IgG2b

 

OVPA8

January 2020

High-grade serous ovarian carcinoma (HGSOC) is the most frequent histological type of ovarian cancer and the one with worst clinical prognosis. The majority of established ovarian cancer cell lines used in the research have unclear histological origin and probably do not represent HGSOC. New and reliable cellular models of HGSOC are needed. A new cell line, OVPA8, has been established and characterized.

This newly-established OVPA8 cell line shows morphologic and genetic features consistent with HGSOC, such as epithelial morphology, multiple chromosomal aberrations, TP53 mutation, BRCA1 mutation, and loss of one copy of BRCA2. The OVPA8 line has a stable STR profile. Cells are positive for EpCAM, CK19, and CD44 and have a relatively low plating efficiency, ability to form spheroids, a low migration rate, and intermediate invasiveness in Matrigel™, compared to other ovarian cancer lines. OVPA8 is sensitive to paclitaxel and resistant to cisplatin and can be a valuable preclinical model for studies on high-grade serous ovarian cancer.    

Catalogue number	Cell line name	Keywords
19062601	OVPA8	OVPA8, Ovarian cancer, High-grade serous ovarian cancer, HGSOC, TP53 mutation, BRCA1 mutation

 

BB2

January 2020 

The monoclonal antibody BBE, alongside TYG 5 are antibodies developed to identify a 10-amino acid sequence from the major structural protein of the Saccharomyces cerevisiae Tyl virus-like particle. These two sequences have overlapping binding sites, which is useful when used in combination for double labelling in immunofluorescence or immunoelectron microscopy. BB2 does not cross-react with trypanosomal proteins.  

Catalogue number	Cell line name	Keywords
19072501	BB2	Anti-TY, T.brucei, virus like particle

 

CD1B4

January 2020

The centriole is a feature of eukaryotic cells that construct cilia or flagella and is unusual in that it is one of the few organelles which exist in a single copy. In G1, a cell has two centrioles, one is mature and was generated at least two cell cycles ago. The other was produced in the previous cell cycle and is immature. Both centrioles then nucleate one procentriole each which subsequently elongate to full-length centrioles, usually in S or G2 phase.

The CD1B4 antibody has been used to specifically detect one centriole in the centriole pair, specifically the mature centriole. This epitope has been identified as a 96-kD protein termed “Cenexin”.  This antibody stains the mature centriole regardless of whether it is forming a cilium and it stains a single centriole in cells which do not possess a primary cilia. 

Catalogue number	Cell line name	Keywords
19072502	CD1B4	Cenexin, mature mammalian centrioles, ODF

 

L1C6

January 2020 

The African parasite Trypanosoma brucei causes African sleeping sickness in cattle and humans. Trypanosomes possess a single flagellum that is attached to their cell body via the flagellum attachment zone (FAZ). There is a complex transmembrane crosslinking of this FAZ to the paraflagellar rod (PFR) and axoneme within the flagellum. Using the L1C6 antibody (amongst others developed in the Sir William Dunn School of Pathology, Oxford) it has been shown that although the growth of both cytoplasmic FAZ filament and external paraflagellar rod (PFR) are related, their growth initiates at different time points during the cell cycle and the two structures elongate at distinct rates.

Catalogue number	Cell line name	Keywords
19072504	L1C6	Nucleolus, T.brucei, trypanosomes

 

HT29 drug resistant clones

December 2019

Mucins are increasingly implicated as playing a role in the development of colon cancer and the resistance of tumour cells to chemotherapy. Distinct sub-populations of mucus-secreting cells have been obtained from the colon cancer cell line HT-29 after long-term treatment with the anti-cancer drugs, methotrexate (MTX) and 5-fluorouracil (5-FU), (Lesuffleur et.al, 1990, Lesuffleur et.al, 1991, respectively).

Catalogue number	Cell line name	Keywords
15121701	HT29-5F7	HT29-5F7, 5-fluorouracil and methotrexate-resistance, enterocytic cells, MUC1 mucin
15121702	HT29-5F12	HT29-5F12, 5-fluorouracil -resistance, enterocytic cells, MUC2 mucin
15121706	HT29-5M21	HT29-5M21, methotrexate-resistance, clone, MUC5AC and MUC5B
15121707	HT29-5M12	HT29-5M12, 5-fluorouraci, methotrexate-resistance, clone, mucin, MUC5B

 

HipSci 

October 2019

A key component of the Human Induced Pluripotent Stem Cells Initiative (HipSci) is the systematic generation of iPSCs from hundreds of donors using a standardised experimental pipeline.  The iPSC lines are generated from hundreds of healthy donors, plus several cohorts of donors with inherited genetic diseases. The use of cell lines from phenotypically normal donors allows the study of how common genetic variations affect the cellular phenotypes. 

Catalogue number	Cell line name	Disease Status
77650803	HPSI1014i-xejd_1	Normal
77650633	HPSI0616i-biln_1	Hereditary spastic paraplegia
77650800	HPSI0115i-rehy_2	Normal
77650479	HPSI0416i-mefc_1	Usher syndrome and congenital eye defects
77650639	HPSI0416i-mapx_1	Alport syndrome
77650542	HPSI0516i-oadp_5	Kabuki syndrome
77650653	HPSI0616i-giql_1	Bleeding and platelet disorders
77650858	HPSI0115i-payf_1	Normal
77650655	HPSI0616i-gayk_3	Bleeding and platelet disorders

 

HipSci

December 2019

A key component of the Human Induced Pluripotent Stem Cells Initiative (HipSci) is the systematic generation of iPSCs from hundreds of donors using a standardised experimental pipeline.  The iPSC lines are generated from hundreds of healthy donors, plus several cohorts of donors with inherited genetic diseases. The use of cell lines from phenotypically normal donors allows the study of how common genetic variations affect the cellular phenotypes. 

Catalogue number	Cell line name	Disease status
77650823	HPSI1116i-zehh_3	Rare genetic neurological disorder
77650824	HPSI1116i-zehh_4	Rare genetic neurological disorder
77650826	HPSI1016i-eoxu_2	Rare genetic neurological disorder
77650827	HPSI1016i-eoxu_6	Rare genetic neurological disorder
77650793	HPSI1016i-livl_2	Rare genetic neurological disorder
77650794	HPSI1016i-livl_5	Rare genetic neurological disorder
77650795	HPSI1016i-riwg_2	Rare genetic neurological disorder
77650796	HPSI1016i-riwg_8	Rare genetic neurological disorder

 

PEO1 CDDP

January 2019

The PEO1-CDDP Cell Line is an in-vitro model to understand the changes in EGF signalling after the onset of cisplatin resistance. Coupled with this change in drug sensitivity, this model also has a modified responsiveness to ligands of the erbB receptor family. This model will be helpful in studying the changes in cellular signalling after drug treatment, which are currently attracting a great deal of interest and this model will be of particular relevance when cytotoxic therapy is combined with signalling inhibitors.

Catalogue number	Cell line name	Keywords
16012001	PEO1-CDDP	Human ovarian cancer; oestrogen receptor, drug resistance, cisplatin resistance

 

Immortalised mouse embryonic fibroblast cell lines

January 2019

The research group led by Professor Catrin Pritchard of University of Leicester, UK, has developed a series of transgenic mouse models containing knockout, inactivating or oncogenic mutations in the c-raf gene. The c-raf protein has the ability to activate the RAS/RAF/MEK/ERK signal transduction cascade. The RAS/RAF/MEK/ERK cascade is a conserved intracellular signalling pathway that plays a crucial role in controlling the ability of cells to respond to their environment. These investigators have derived immortalised mouse embryonic fibroblast cell lines from these transgenic mice.  These derived cell lines provide in vitro models to assess the role of mutations in c-Raf1 in response to apoptotic stimuli and their capability to respond to other external stimuli via the RAS/RAF/MEK/ERK intracellular signalling pathway.   

Catalogue number	Cell line name	Keywords
16012201	C-Raf KI D486A	Knock in, oncogenic mutant
16012205	MEF Raf1 FF/FF KI	Knock-in, FF/FF Raf1 (Y340F-Y341F) mutant, inactivation
16012209	MEF RAF1 KO	Knock-out, RAF1, mutant, inactivation

 

AMEF OGG1 KO

January 2019

DNA damage generated from the byproducts of oxidative cellular metabolism have been proposed as a key factor in cancer and aging. Oxygen free radicals cause predominantly base damage in DNA with the most frequent mutagenic base lesion being 7,8-dihydro-8- oxoguanine (8-oxoG). This altered base lesion can pair with adenosine as well as cytosine residues and can lead to a greatly increased frequency of spontaneous GzC3TzA transversion mutations in repair-deficient bacterial and yeast cells. Eukaryotic cells use a specific DNA glycosylase, the product of the OGG1 gene, to remove 8-oxoG base lesions from DNA.  To assess the role of the mammalian enzyme in repair of DNA damage and prevention of carcinogenesis, homozygous ogg12y2 null mice have been generated. These animals are viable and accumulate abnormal levels of 8-oxoG in their genomes. Mouse embryonic fibroblast cell lines MEFs derived from ogg1 null mice have been established by spontaneous immortalisation in culture for the study of mutation rates in the absence of the DNA glycosylase. 

Catalogue number	Cell line name	Keywords
16022531	MEF OGG1 KO	Mouse embryonic fibroblast, Ogg1 mutant

 

LF2

January 2019

Embryonic stem cells provide a powerful system for the experimental analysis of mammalian development. Their genetic manipulation allows the introduction of defined modifications into the mouse germ-line, whilst in culture they constitute both an assay system for, and a potential source of, embryonic regulatory factors. Non-feeder culture conditions have been developed for the propagation of this cell line (LF2) in an undifferentiated state.    

Catalogue number	Cell line name	Keywords
16022532	LF2	Mouse embryonic stem cell

 

Human colorectal carcinoma cell lines

January 2019

HCT 116 BRCA2 -/- [42] and [46] human colorectal carcinoma cell lines were developed to study the role of BRCA2 in DNA repair and to identify additional functions of the BRCA2 gene. These cells exhibit phenotypes consistent with the loss of Rad51 foci in the presence of double-strand breaks, chromosomal rearrangements and show an elevated sensitivity to the DNA-damaging agents. They will also be useful to identify potential therapeutic agents in cancer cells with BRCA2 mutations.   

Catalogue number	Cell line name	Keywords
16071901	HCT 116 BRCA2 -/- [42]	BRCA2 Knock-Out, colon carcinoma model, HCT116, HCT 116 BRCA2 KO [42]
18061301	HCT 116 BRCA2 -/- [46]	BRCA2 Knock-Out, colon carcinoma model, HCT116

 

MCF7 AREc32

January 2019

This stable human mammary MCF7-derived reporter cell line called AREc32 contains copies of the rat Glutathione-S-transferase (GST) antioxidant response element (ARE) linked to a luciferase gene. Antioxidant response elements (AREs) are transcriptional regulatory sequences involved in the activation of genes coding for a number of antioxidant proteins and enzymes that work in concert to protect tissues from oxidative insults. This cell line can be used to investigate whether anticancer drugs can induce ARE-driven gene expression. Measurement of redox-potential and antioxidant activity can also be used to monitor anti-inflammatory activity and assess water quality in aquatic environments 

Catalogue number	Cell line name	Keywords
16071902	MCF7 AREc32	Cancer; drug metabolism, Antioxidant Response Element (ARE)- Luciferase

 

S_N40R2 and N_N20R1

January 2019

The tumour suppressor protein encoded by the TP53 gene is activated in response to a diverse range of cellular stresses which can lead to cell cycle arrest and cell death. This activation process is tightly controlled by an auto-regulatory feedback loop involving a direct protein-protein binding interaction between p53 and the product of the Murine Double Minute 2 (MDM2) oncogene. MDM2 is an E3 ubiquitin ligase and is a target gene of the p53 gene. MDM2 is a key negative regulator of p53 protein activity.  Once bound to p53, MDM2 inhibits p53-controlled transcriptional (RNA) activity and it also ubiquitinates the p53 protein for nuclear export and degradation by the proteosome. The reactivation of wild-type p53 (non-mutated) following exposure to small selective antagonists of the MDM2/p53 binding interaction is an attractive treatment strategy in tumours containing wild type p53. To determine how resistance to MDM2/p53 binding antagonists might develop, osteosarcoma cells, SJSA-1, and neuroblastoma cells, NGP, have been exposed to growth inhibitory concentrations of the chemically distinct MDM2 inhibitor, Nutlin-3,  and resistant cell lines have been derived, S_N40R2 and N_N20R1.  An analysis of the resistant cell strains indicates that MDM2/p53 binding antagonists will select for p53 mutations present in tumours present at a low frequency at initial diagnosis, leading to resistance cells.  Tumours and antagonist-resistant cell lines will remain responsive to other therapies, including ionising radiation. 

Catalogue number	Cell line name	Keywords
17011210	S_N40R2	MDM2 inhibitor, Nutlin-3, resistance, MDM2-application
17011212	N_N20R1	MDM2 inhibitor, Nutlin-3, resistance, MDM2-application

  

HCT 116 p300 cell lines

January 2019

The transcriptional cofactor and prototype histone acetyltransferase, p300, is involved in regulating multiple cellular processes including cell cycle regulation, proliferation, differentiation, apoptosis, DNA damage repair and adhesion properties. Somatic inactivating mutations of p300 are associated with several cancers including breast, colorectal and gastric cancers.

The research group led by Professor G Murphy, Cambridge University, has generated a series of p300-deficient clones from the colon carcinoma cell line, HCT 116 by gene targeting. A comparison of epithelial and mesenchymal-related proteins in p300-deficient and parental HCT 116 cells has shown that a number of genes involved in the interactions between cell plasma membrane and extracellular matrix that are typically involved in the process of  ‘epithelial to mesenchyme transition’ are differentially regulated at both the RNA and protein level. Cells deficient in p300 function were shown to have aggressive ‘cancer’ phenotypes, with loss of cell–cell adhesion, defects in cell–matrix adhesion and increased rates of migration through collagen and Matrigel™ matrices.

These HCT 116 p300 KO cell Lines are tools for the in-vitro study of p300 in colon carcinoma, and specifically of its role in p53-dependent apoptosis, cellular adhesion and migration. Cell lines available are p300 WT (HCT 116),  p300 KO [D10] (HCT 116), p300 KO [F5] (HCT 116), p300 KO [F2] (HCT 116) and p300 KO Rescue (HCT 116). Experiments conducted should be using at least 2 of the 3 KO cell lines. The KO clones are D10 and F5 are the most characterised. It is recommended that the parental cell line (WT) is used as the reference when using these knockout cell lines.

 

Catalogue number	Cell line name	Keywords
17011201	17011201 HCT 116 p300 KO [D10]	p300 Knock-Out, colon carcinoma model, HCT 116, p53-dependent apoptosis, cellular adhesion and migration
17011211	HCT 116 p300 KO Rescue	p300 Knock-Out, colon carcinoma model, HCT 116, p53-dependent apoptosis, cellular adhesion and migration
17011214	HCT 116 p300 WT	p300 Knock-Out, colon carcinoma model, HCT 116, p53-dependent apoptosis, cellular adhesion and migration
17011215	HCT 116 p300 KO [F2]	p300 Knock-Out, colon carcinoma model, HCT 116, p53-dependent apoptosis, cellular adhesion and migration
17011216	HCT 116 p300 KO [F5]	p300 Knock-Out, colon carcinoma model, HCT 116, p53-dependent apoptosis, cellular adhesion and migration

 

OX-47

January 2019

The hybridoma cell line OX-47 secretes mouse monoclonal IgG1 antibody reactive with rat CD147 membrane protein. This antibody binds to activated rat T-lymphocytes.  It reacts to all T and B cell blasts strongly. It gives weak labelling to thoracic duct lymphocytes and thymocytes. The antibody labels vascular endothelium, kidney tubules and brain capillaries.

Catalogue number	Cell line name
15090220	OX-47

 

HipSci Panels

April 2017

For new users of our iPSC resource, we have composed two panels of lines from the HipSci iPSC collection that we would recommend. These lines have been selected based on their high pluritest scores, low CNV’s, comprehensively characterised assay data, and their ability to differentiate.

These lines are also available to order as individual lines.

 

Catalogue number	Name	Keywords
77659901	HipSci feeder free panel	Normal, Mixed
77659933	HipSci feeder free panel	Normal, Mixed

 

Nd ES

January 2017

A mouse embryonic stem cell (ES) line (Nd) containing a novel fluorescent reporter (VNP) to investigate the temporal dynamics of Nanog expression in mouse and to dissect the lineage potential at different Nanog expression levels. This novel reporter ES cell line allows the accurate monitoring the dynamic expression of the transcription factor Nanog in the pluripotent state. The cell line will be a valuable tool to obtain quantitative measurements of global gene expression in pluripotent mouse ES cells in different states, allowing a detailed molecular mapping of the pluripotent state.

Catalogue number	Cell line name	Keywords
16010601	Nd ES	mouse embryonic stem cells, Nanog expression, pluripotency

 

 

CHO lines

A number of new CHO cell line variants, developed in the laboratory of Dr Mike Clark at Cambridge University have been deposited with the ECACC General Collection, by Cambridge Enterprise. These CHO cell lines express different allotypes of Fc gamma RIIA receptors on the cell surface and are useful for the investigating the binding of recombinant antibodies to human Fc receptors. Cell lines expressing human and macaque Fc receptors have been developed since these are both useful to pre-clinical research. The cell lines may be used for the measurement, by flow cytometry, of monomeric IgG binding or complexed  IgG binding to receptors. They are also used for studying the association of Fc receptor bearing cell lines with red blood cells coated with IgG. They are used in assays that include the target cell of the IgG, comparing natural IgG constant regions, mutated IgG constant regions and those with altered carbohydrate profiles. The cell lines may also be used as a release assay for batches of clinical grade antibodies since binding will be sensitive to aggregates or changes in carbohydrate composition.

Catalogue number	Cell line name	Keywords
15042901	CHO-K1.Cl6	CHO-K1, FcγRIIIa , Fc gamma receptor 3A, 158V, allotype, human IgG , IgG binding
15042902	CHO-K1.Cl7	CHO-K1, FcγRIIIa , Fc gamma receptor 3A, 158F, allotype, human IgG , IgG binding
15042903	CHO-K1.Cl-0204	CHO-K1, FcγRIIa , Fc gamma receptor 2A, 131R, allotype
15042905	CHO-K1.Cl-0205	CHO-K1, FcγRIIa , Fc gamma receptor 2A, 131H, allotype, human IgG , IgG binding
15042907	CHO-K1.Cl-0206	CHO-K1, FcγRIIb , Fc gamma receptor 2b
15042908	CHO-K1.Cl-0235	CHO-K1, non-human primate FcγRIIIa , Fc gamma receptor 3A, Pan troglodytes, chimpanzee
15042909	CHO-K1.Cl-0236 / CHO-K1.Cl-0239	CHO-K1, non-human primate FcγRIIIa , Fc gamma receptor 3A, Cercocebus torquatus, red-crowned mangabey, papio anubis, olive baboon
15042910	CHO-K1.Cl-0237	CHO-K1, non-human primate FcγRIIIa , Fc gamma receptor 3A, Macaca fascicularis (Cynomolgus macaque)
15042911	CHO-K1.Cl-0238	CHO-K1, non-human primate FcγRIIIa , Fc gamma receptor 3A, Macaca mulatta (Rhesus macaque)
15042912	CHO-K1.Cl-0270	CHO-K1, non-human primate FcγRIIb , Fc gamma receptor 2b, Macaca mulatta, Rhesus macaque, Macaca fascicularis, Cynomolgus macaque

 

L929/A (an Adriamycin-resistant cell line)

This adriamycin-resistant cell line has been developed by exposure of the parent L929 murine fibroblast cell line (ECACC catalogue no. 85011425) to increasing concentrations of adriamycin in vitro. L929/A cells can be used in the development of novel anti-cancer treatments. The parent cell line L929 was derived from normal subcutaneous areolar adipose tissue.

Catalogue No.	Cell line name	Keywords
14112101	L929/A	Mouse C3H/An connective tissue Adriamycin resistant

 

 

 

A Reporter Cell Line for Use in Autophagy Studies

A stable cell line expressing EGFP-tagged LC3 (rat) in background of HEK293 cell line. This reporter cell line can be used to monitor induction of autophagy after amino acid starvation or rapamycin treatment as well as autophagosome formation. Also, this cell line provides a useful tool for the study of autophagy using GFP-LC3 as a standard assay read out by both immunofluorescence and biochemical methods.

Catalogue No.	Cell line name	Keywords
14050801	HEK293A GFP-LC3	Human embryonic kidney, autophagy, HEK293, EGFP-rat LC3, reporter cell line, ATG8, microtubule-associated protein 1 light chain 3 beta (MAP1LC3B)

 

 

HeLa-Mitotrap ‘Knock-sideways’ Cell Lines for Investigating Protein Function

Two new HeLa-Mitotrap cell lines, which can be used to determine the function of a protein, by rapidly inactivating the protein of interest through rapamycin induced rerouting to the mitochondria, a ‘knock sideways’ approach, are now available from ECACC. This method of protein inactivation, which relocates proteins away from their site of action rather than destroying them, is more rapid than knockout or knock down strategies.

 

Catalogue No.	Cell line name	Keywords
15042201	HeLa-Mitrotrap	Human cervix carcinoma, knocksideways, rapid protein inactivation by rerouting to mitochondria for functional studies, genetically modified
15042203	HeLa-Mitrotrap Ap1g1-FKBP	Human cervix carcinoma, knocksideways, rapid protein inactivation by rerouting to mitochondria for functional studies, adaptor protein (Ap1g1), genetically modified

 

 

ULK cell lines for autophagy research

A series of mouse embryonic fibroblasts with different capability for  uncoordinated (Unc)-51-like kinase 1 and 2 (ULK1 and ULK2) activity, which are known to play a critical role during the activation of autophagy, are now available from PHE’s European Collection of Authenticated Cell Cultures (ECACC).  Autophagy is the mechanism by which a cell degrades unnecessary or dysfunctional cellular components and has been implicated in several medical scenarios such as cancer, neurodegeneration and immunity related disorders.  The cell lines were deposited by Dr Sharon Tooze of Cancer Research UK.

Catalogue No.	Cell line name	ULK 1 and 2 status	Immortalisation method
14050808	ULK1/2 WT MEF (SIM)	Wild type	Spontaneously immortalised
14050804	ULK1/2 WT MEF (SV40)	Wild type	SV40-immortalised
14050806	ULK1 KO MEF (SIM)	ULK1 knock-out	Spontaneously immortalised
14050807	ULK1 KO MEF (SV40)	ULK1 knock-out	SV40-immortalised
14050810	ULK2 KO MEF (SIM)	ULK2 knock-out	Spontaneously immortalised
14050805	ULK2 KO MEF (SV40)	ULK2 knock-out	SV40-immortalised
14050803	MEF Ulk1 -/- Ulk2 -/- (DKO) (SIM)	Double knock-out	Spontaneously immortalised
14050802	MEF Ulk1 -/- Ulk2 -/- (DKO) (SV40)	Double knock-out	SV40-immortalised